Regional localization and characterization of a DNA segment on the long arm of chromosome 21

D. N. Cooper; S. C. Niemann; J. R. Gosden; A. R. Mitchell; A. M. Goate; G. S. Rajendran; D. A. Miller; L. Lim; J. Schmidtke

doi:10.1007/BF00591073

Regional localization and characterization of a DNA segment on the long arm of chromosome 21

D. N. Cooper, S. C. Niemann, J. R. Gosden, A. R. Mitchell, A. M. Goate, G. S. Rajendran, D. A. Miller, L. Lim, J. Schmidtke

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A human genomic DNA fragment, pAM37 (HGM8; D21S22), was mapped to chromosome 21q2.1-q2.21 by in situ hybridization. This segment is therefore situated on the boundary of the "pathological region" of Down syndrome. A genomic restriction map encompassing 35 kb of chromosome 21 was derived and two restriction fragment length polymorphisms (RFLPs) were mapped and characterized. A homologous sequence was detected in the mouse genome but no homologous RNA was detected in a range of human tissues. This DNA segment will contribute to the linkage mapping of chromosome 21 and will facilitate delineation of the pathological region of Down syndrome.

Original language	English
Pages (from-to)	129-135
Number of pages	7
Journal	Human Genetics
Volume	75
Issue number	2
DOIs	https://doi.org/10.1007/BF00591073
State	Published - Feb 1987
Externally published	Yes

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title = "Regional localization and characterization of a DNA segment on the long arm of chromosome 21",

abstract = "A human genomic DNA fragment, pAM37 (HGM8; D21S22), was mapped to chromosome 21q2.1-q2.21 by in situ hybridization. This segment is therefore situated on the boundary of the {"}pathological region{"} of Down syndrome. A genomic restriction map encompassing 35 kb of chromosome 21 was derived and two restriction fragment length polymorphisms (RFLPs) were mapped and characterized. A homologous sequence was detected in the mouse genome but no homologous RNA was detected in a range of human tissues. This DNA segment will contribute to the linkage mapping of chromosome 21 and will facilitate delineation of the pathological region of Down syndrome.",

author = "Cooper, {D. N.} and Niemann, {S. C.} and Gosden, {J. R.} and Mitchell, {A. R.} and Goate, {A. M.} and Rajendran, {G. S.} and Miller, {D. A.} and L. Lim and J. Schmidtke",

year = "1987",

month = feb,

doi = "10.1007/BF00591073",

language = "English",

volume = "75",

pages = "129--135",

journal = "Human Genetics",

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T1 - Regional localization and characterization of a DNA segment on the long arm of chromosome 21

AU - Cooper, D. N.

AU - Niemann, S. C.

AU - Gosden, J. R.

AU - Mitchell, A. R.

AU - Goate, A. M.

AU - Rajendran, G. S.

AU - Miller, D. A.

AU - Lim, L.

AU - Schmidtke, J.

PY - 1987/2

Y1 - 1987/2

N2 - A human genomic DNA fragment, pAM37 (HGM8; D21S22), was mapped to chromosome 21q2.1-q2.21 by in situ hybridization. This segment is therefore situated on the boundary of the "pathological region" of Down syndrome. A genomic restriction map encompassing 35 kb of chromosome 21 was derived and two restriction fragment length polymorphisms (RFLPs) were mapped and characterized. A homologous sequence was detected in the mouse genome but no homologous RNA was detected in a range of human tissues. This DNA segment will contribute to the linkage mapping of chromosome 21 and will facilitate delineation of the pathological region of Down syndrome.

AB - A human genomic DNA fragment, pAM37 (HGM8; D21S22), was mapped to chromosome 21q2.1-q2.21 by in situ hybridization. This segment is therefore situated on the boundary of the "pathological region" of Down syndrome. A genomic restriction map encompassing 35 kb of chromosome 21 was derived and two restriction fragment length polymorphisms (RFLPs) were mapped and characterized. A homologous sequence was detected in the mouse genome but no homologous RNA was detected in a range of human tissues. This DNA segment will contribute to the linkage mapping of chromosome 21 and will facilitate delineation of the pathological region of Down syndrome.

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VL - 75

SP - 129

EP - 135

JO - Human Genetics

JF - Human Genetics

IS - 2

ER -

Regional localization and characterization of a DNA segment on the long arm of chromosome 21

Abstract

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