Regional distribution of neuropeptide processing endopeptidases in adult rat brain

Y. L. Berman, A. K. Rattan, K. Carr, L. Devi

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Many peptide hormone and neuropeptide precursors undergo post-translational processing at mono- and/or dibasic residues. An enzymatic activity capable of processing prodynorphin at a monobasic processing site designated 'dynorphin converting enzyme' has been previously reported in rat brain and bovine pituitary. In this study the distribution of dynorphin converting enzyme activity in ten regions of rat brain has been compared with the distribution of subtilisin-like processing enzymes and with the immuno-reactive dynorphin peptides. The distribution of dynorphin converting enzyme activity generally matches the distribution of immuno-reactive dynorphin B-13 in most but not all brain regions. The regions that are known to have a relatively large number of immuno-reactive dynorphin-neurons also contain high levels of dynorphin converting enzyme activity. The distribution of dynorphin converting enzyme activity does not match the distribution of subtilisin-like processing enzyme or carboxypeptidase E activities. Taken together the data support the possibility that the dynorphin converting enzyme is involved in the maturation of dynorphin, as well as other neuropeptides, and peptide hormones.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalBiochimie
Volume76
Issue number3-4
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • carboxypeptidase E
  • endorphin
  • enkephalin
  • neuropeptide biosynthesis
  • opioid peptide

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