Reduced expression of complexins I and II in rats bred for learned helplessness

Mathias Zink; Barbara Vollmayr; Peter J. Gebicke-Haerter; Fritz A. Henn; Johannes Thome

doi:10.1016/j.brainres.2007.01.066

Reduced expression of complexins I and II in rats bred for learned helplessness

Mathias Zink
, Barbara Vollmayr
, Peter J. Gebicke-Haerter
, Fritz A. Henn
, Johannes Thome

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Disturbed synaptic transmission contributes to the pathophysiology of mood disorders. Post mortem studies reported reduced expression of the synaptic vesicle protein (SVP) complexins I and II in depression. Antidepressants were found to induce the expression of these genes. Since animals with congenital susceptibility to learned helplessness provide a valid animal model of depression, we investigated the expression of different SVPs in this system by semiquantitative in situ hybridization. Rats bred for congenital learned helpless behavior (cLH, N = 6) failed to interrupt foot shock currents by lever pressing (mean 12.3 failures out of 15 trials). These animals showed significantly lower expression of complexins I and II mRNA in hippocampal, limbic and cortical brain areas compared to not helpless animals (cNLH, N = 6) with a mean failure rate of 0.83 out of 15 trials. Expression levels of complexins I and II significantly correlated with the failure rate in the test paradigm. In contrast, the expressions of synaptotagmin I and synaptophysin were found unchanged. This investigation provides a further validation of the LH model of depression. The experimental data fit well into current pathogenetic concepts of mood disorders and support the hypothesis, that complexins are pivotal players in the pathophysiology of depression and tentative targets of antidepressants.

Original language	English
Pages (from-to)	202-208
Number of pages	7
Journal	Brain Research
Volume	1144
Issue number	1
DOIs	https://doi.org/10.1016/j.brainres.2007.01.066
State	Published - 4 May 2007
Externally published	Yes

Keywords

Animal model
Complexin
Depression
Learned helplessness
Synapse

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10.1016/j.brainres.2007.01.066

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title = "Reduced expression of complexins I and II in rats bred for learned helplessness",

abstract = "Disturbed synaptic transmission contributes to the pathophysiology of mood disorders. Post mortem studies reported reduced expression of the synaptic vesicle protein (SVP) complexins I and II in depression. Antidepressants were found to induce the expression of these genes. Since animals with congenital susceptibility to learned helplessness provide a valid animal model of depression, we investigated the expression of different SVPs in this system by semiquantitative in situ hybridization. Rats bred for congenital learned helpless behavior (cLH, N = 6) failed to interrupt foot shock currents by lever pressing (mean 12.3 failures out of 15 trials). These animals showed significantly lower expression of complexins I and II mRNA in hippocampal, limbic and cortical brain areas compared to not helpless animals (cNLH, N = 6) with a mean failure rate of 0.83 out of 15 trials. Expression levels of complexins I and II significantly correlated with the failure rate in the test paradigm. In contrast, the expressions of synaptotagmin I and synaptophysin were found unchanged. This investigation provides a further validation of the LH model of depression. The experimental data fit well into current pathogenetic concepts of mood disorders and support the hypothesis, that complexins are pivotal players in the pathophysiology of depression and tentative targets of antidepressants.",

keywords = "Animal model, Complexin, Depression, Learned helplessness, Synapse",

author = "Mathias Zink and Barbara Vollmayr and Gebicke-Haerter, \{Peter J.\} and Henn, \{Fritz A.\} and Johannes Thome",

note = "Funding Information: This study was funded by the Deutsche Forschungs-gemeinschaft (SFB 636 B2). The excellent technical assistance of H. Schamber and A. Tredup is greatly acknowledged.",

year = "2007",

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doi = "10.1016/j.brainres.2007.01.066",

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T1 - Reduced expression of complexins I and II in rats bred for learned helplessness

AU - Zink, Mathias

AU - Vollmayr, Barbara

AU - Gebicke-Haerter, Peter J.

AU - Henn, Fritz A.

AU - Thome, Johannes

N1 - Funding Information: This study was funded by the Deutsche Forschungs-gemeinschaft (SFB 636 B2). The excellent technical assistance of H. Schamber and A. Tredup is greatly acknowledged.

PY - 2007/5/4

Y1 - 2007/5/4

N2 - Disturbed synaptic transmission contributes to the pathophysiology of mood disorders. Post mortem studies reported reduced expression of the synaptic vesicle protein (SVP) complexins I and II in depression. Antidepressants were found to induce the expression of these genes. Since animals with congenital susceptibility to learned helplessness provide a valid animal model of depression, we investigated the expression of different SVPs in this system by semiquantitative in situ hybridization. Rats bred for congenital learned helpless behavior (cLH, N = 6) failed to interrupt foot shock currents by lever pressing (mean 12.3 failures out of 15 trials). These animals showed significantly lower expression of complexins I and II mRNA in hippocampal, limbic and cortical brain areas compared to not helpless animals (cNLH, N = 6) with a mean failure rate of 0.83 out of 15 trials. Expression levels of complexins I and II significantly correlated with the failure rate in the test paradigm. In contrast, the expressions of synaptotagmin I and synaptophysin were found unchanged. This investigation provides a further validation of the LH model of depression. The experimental data fit well into current pathogenetic concepts of mood disorders and support the hypothesis, that complexins are pivotal players in the pathophysiology of depression and tentative targets of antidepressants.

AB - Disturbed synaptic transmission contributes to the pathophysiology of mood disorders. Post mortem studies reported reduced expression of the synaptic vesicle protein (SVP) complexins I and II in depression. Antidepressants were found to induce the expression of these genes. Since animals with congenital susceptibility to learned helplessness provide a valid animal model of depression, we investigated the expression of different SVPs in this system by semiquantitative in situ hybridization. Rats bred for congenital learned helpless behavior (cLH, N = 6) failed to interrupt foot shock currents by lever pressing (mean 12.3 failures out of 15 trials). These animals showed significantly lower expression of complexins I and II mRNA in hippocampal, limbic and cortical brain areas compared to not helpless animals (cNLH, N = 6) with a mean failure rate of 0.83 out of 15 trials. Expression levels of complexins I and II significantly correlated with the failure rate in the test paradigm. In contrast, the expressions of synaptotagmin I and synaptophysin were found unchanged. This investigation provides a further validation of the LH model of depression. The experimental data fit well into current pathogenetic concepts of mood disorders and support the hypothesis, that complexins are pivotal players in the pathophysiology of depression and tentative targets of antidepressants.

KW - Animal model

KW - Complexin

KW - Depression

KW - Learned helplessness

KW - Synapse

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DO - 10.1016/j.brainres.2007.01.066

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ER -

Reduced expression of complexins I and II in rats bred for learned helplessness

Abstract

Keywords

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