Redistribution of endosomal membranes to the African swine fever virus replication site

Miguel Ángel Cuesta-Geijo, Lucía Barrado-Gil, Inmaculada Galindo, Raquel Muñoz-Moreno, Covadonga Alonso

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

African swine fever virus (ASFV) infection causes endosomal reorganization. Here, we show that the virus causes endosomal congregation close to the nucleus as the infection progresses, which is necessary to build a compact viral replication organelle. ASFV enters the cell by the endosomal pathway and reaches multivesicular late endosomes. Upon uncoating and fusion, the virus should exit to the cytosol to start replication. ASFV remodels endosomal traffic and redistributes endosomal membranes to the viral replication site. Virus replication also depends on endosomal membrane phosphoinositides (PtdIns) synthesized by PIKfyve. Endosomes could act as platforms providing membranes and PtdIns, necessary for ASFV replication. Our study has revealed that ASFV reorganizes endosome dynamics, in order to ensure a productive infection.

Original languageEnglish
Article number133
JournalViruses
Volume9
Issue number6
DOIs
StatePublished - Jun 2017
Externally publishedYes

Keywords

  • ASF viral factory
  • African swine fever virus (ASFV)
  • Endosomal pathway
  • Endosomes
  • Microtubules
  • Phosphatidylinositol kinases
  • Phosphoinositides

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