Red algal sulfated galactan binds and protects neural cells from hiv-1 gp120 and tat

Vitor H. Pomin, Fakhri Mahdi, Weihua Jin, Fuming Zhang, Robert J. Linhardt, Jason J. Paris

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The potential neuroprotective capacity of four different sulfated glycans: Botryocladia occidentalis-derived sulfated galactan (BoSG) (MW > 100 kDa), Lytechinus variegatus-derived sulfated fucan (LvSF) (MW~90 kDa), high-molecular weight dextran sulfate (DxS) (MW 100 kDa), and unfractionated heparin (UFH) (MW~15 kDa), was assessed in response to the HIV-1 proteins, R5-tropic glycoprotein 120 (gp120) and/or trans-activator of transcription (Tat), using primary murine neurons co-cultured with mixed glia. Compared to control-treated cells in which HIV-1 proteins alone or combined were neurotoxic, BoSG was, among the four tested sulfated glycans, the only one capable of showing significant concentration-dependent neuroprotection against Tat and/or gp120, alone or combined. Surface plasmon resonance-based data indicate that BoSG can bind both HIV-1 proteins at nM concentrations with preference for Tat (7.5 × 10−8 M) over gp120 (3.2 × 10−7 M) as compared to UFH, which bound gp120 (8.7 × 10−7 M) over Tat (5.7 × 10−6 M). Overall, these data support the notion that sulfated glycan extracted from the red alga B. occidentalis, BoSG, can exert neuroprotection against HIV-1 Tat and gp120, potentially via direct molecular interactions.

Original languageEnglish
Article number714
JournalPharmaceuticals
Volume14
Issue number8
DOIs
StatePublished - Aug 2021
Externally publishedYes

Keywords

  • Glycoprotein 120
  • HIV
  • Neuroprotection
  • Sulfated glycan
  • Surface plasmon resonance
  • Trans-activating transcriptor

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