Recomendaciones para la selección de antimicrobianos en el estudio de la sensibilidad in vitro con sistemas automáticos y semiautomáticos

Translated title of the contribution: Recommendations for selecting antimicrobial agents for in vitro susceptibility studies using automatic and semiautomatic systems

Rafael Cantón, Juan Ignacio Alós, Fernando Baquero, Jorge Calvo, José Campos, Javier Castillo, Emilia Cercenado, M. Ángeles Domínguez, Josefina Liñares, Lorena López-Cerezo, Francesc Marco, Beatriz Mirelis, María Isabel Morosini, Ferran Navarro, Antonio Oliver, Emilio Pérez-Trallero, Carmen Torres, Luis Martínez-Martínez, Javier Cobo, María C. FariñasJosé Elías García-Sánchez, Elena Loza, Jesús Oteo, Guillermo Prats, Jesús Rodríguez-Baño, Juan Antonio Sáez-Nieto, Miguel Salavert, Francisco Soriano, Ignacio Trujillano, Fe Tubau, Jordi Vila

Research output: Contribution to journalReview articlepeer-review

16 Scopus citations


The number of clinical microbiology laboratories that have incorporated automatic susceptibility testing devices has increased in recent years. The majority of these systems determine MIC values using microdilution panels or specific cards, with grouping into clinical categories (susceptible, intermediate or resistant) and incorporate expert systems to infer resistance mechanisms. This document presents the recommendations of a group of experts designated by Grupo de Estudio de los Mecanismos de Acción y Resistencia a los Antimicrobianos (GEMARA, Study group on mechanisms of action and resistance to antimicrobial agents) and Mesa Española de Normalización de la Sensibilidad y Resistencia a los Antimicrobianos (MENSURA, Spanish Group for Normalizing Antimicrobial Susceptibility and Antimicrobial Resistance), with the aim of including antimicrobial agents and selecting concentrations for the susceptibility testing panels of automatic systems. The following have been defined: various antimicrobial categories (A: must be included in the study panel; B: inclusion is recommended; and C: inclusion is secondary, but may facilitate interpretative reading of the antibiogram) and groups (0: not used in therapeutics but may facilitate the detection of resistance mechanisms; 1: must be studied and always reported; 2: must be studied and selectively reported; 3: must be studied and reported at a second level; and 4: should be studied in urinary tract pathogens isolated in urine and other specimens). Recommended antimicrobial concentrations are adapted from the breakpoints established by EUCAST, CLSI and MENSURA. This approach will lead to more accurate susceptibility testing results with better detection of resistance mechanisms, and allowing to reach the clinical goal of the antibiogram.

Translated title of the contributionRecommendations for selecting antimicrobial agents for in vitro susceptibility studies using automatic and semiautomatic systems
Original languageSpanish
Pages (from-to)394-400
Number of pages7
JournalEnfermedades Infecciosas y Microbiologia Clinica
Issue number6
StatePublished - 1 Jun 2007
Externally publishedYes


  • Antibiogram
  • Antimicrobials
  • Automatic susceptibility testing systems
  • Concentrations


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