TY - JOUR
T1 - Recombinant influenza A viruses with enhanced levels of PB1 and PA viral protein expression
AU - Belicha-Villanueva, Alan
AU - Rodriguez-Madoz, Juan R.
AU - Maamary, Jad
AU - Baum, Alina
AU - Bernal-Rubio, Dabeiba
AU - de la Escalera, Maria Minguito
AU - Fernandez-Sesma, Ana
AU - García-Sastre, Adolfo
PY - 2012/5
Y1 - 2012/5
N2 - Influenza A viruses containing the promoter mutations G3A/C8U in a given segment express increased levels of the corresponding viral protein during infection due to increased levels of mRNA or cRNA species. The replication of these recombinant viruses is attenuated, and they have an enhanced shedding of noninfectious particles and are incapable of antagonizing interferon (IFN) effectively. Our findings highlight the possibility of increasing influenza virus protein expression and the need for a delicate balance between influenza viral replication, protein expression, and assembly.
AB - Influenza A viruses containing the promoter mutations G3A/C8U in a given segment express increased levels of the corresponding viral protein during infection due to increased levels of mRNA or cRNA species. The replication of these recombinant viruses is attenuated, and they have an enhanced shedding of noninfectious particles and are incapable of antagonizing interferon (IFN) effectively. Our findings highlight the possibility of increasing influenza virus protein expression and the need for a delicate balance between influenza viral replication, protein expression, and assembly.
UR - https://www.scopus.com/pages/publications/84861313040
U2 - 10.1128/JVI.06384-11
DO - 10.1128/JVI.06384-11
M3 - Article
C2 - 22398284
AN - SCOPUS:84861313040
SN - 0022-538X
VL - 86
SP - 5926
EP - 5930
JO - Journal of Virology
JF - Journal of Virology
IS - 10
ER -