Recombinant influenza A viruses with enhanced levels of PB1 and PA viral protein expression

Alan Belicha-Villanueva, Juan R. Rodriguez-Madoz, Jad Maamary, Alina Baum, Dabeiba Bernal-Rubio, Maria Minguito de la Escalera, Ana Fernandez-Sesma, Adolfo García-Sastre

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Influenza A viruses containing the promoter mutations G3A/C8U in a given segment express increased levels of the corresponding viral protein during infection due to increased levels of mRNA or cRNA species. The replication of these recombinant viruses is attenuated, and they have an enhanced shedding of noninfectious particles and are incapable of antagonizing interferon (IFN) effectively. Our findings highlight the possibility of increasing influenza virus protein expression and the need for a delicate balance between influenza viral replication, protein expression, and assembly.

Original languageEnglish
Pages (from-to)5926-5930
Number of pages5
JournalJournal of Virology
Volume86
Issue number10
DOIs
StatePublished - May 2012

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