TY - JOUR
T1 - Recent advances in two-photon imaging
T2 - Technology developments and biomedical applications
AU - Chen, Yu
AU - Guo, Hengchang
AU - Gong, Wei
AU - Qin, Luye
AU - Aleyasin, Hossein
AU - Ratan, Rajiv R.
AU - Cho, Sunghee
AU - Chen, Jianxin
AU - Xie, Shusen
PY - 2013/1
Y1 - 2013/1
N2 - In the past two decades, two-photon microscopy (TPM) transforms biomedicalresearch, allowing nondestructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technologyincluding novel laser sources, new image acquisition paradiams, and microendoscopicimaging systems. Then, we survey the capabilities of TPM imagingof biological relevant molecules such as nicotinamideadenine dinucleotide (NADH), flavin adenine dinucleotide (FAD),and reactive oxygen species (ROS). Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.
AB - In the past two decades, two-photon microscopy (TPM) transforms biomedicalresearch, allowing nondestructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technologyincluding novel laser sources, new image acquisition paradiams, and microendoscopicimaging systems. Then, we survey the capabilities of TPM imagingof biological relevant molecules such as nicotinamideadenine dinucleotide (NADH), flavin adenine dinucleotide (FAD),and reactive oxygen species (ROS). Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.
UR - https://www.scopus.com/pages/publications/84875954081
U2 - 10.3788/COL201311.011703
DO - 10.3788/COL201311.011703
M3 - Article
AN - SCOPUS:84875954081
SN - 1671-7694
VL - 11
JO - Chinese Optics Letters
JF - Chinese Optics Letters
IS - 1
M1 - 011703
ER -