Understanding the formation process of nanoparticles is of the utmost importance to improve their design and production. This especially holds true for self-assembled nanoparticles whose formation processes have been largely overlooked. Herein, we present a new technology that integrates a microfluidic-based nanoparticle synthesis method and Förster resonance energy transfer (FRET) microscopy imaging to visualize nanoparticle self-assembly in real time. Applied to different nanoparticle systems, for example, nanoemulsions, drug-loaded block-copolymer micelles, and nanocrystal-core reconstituted high-density lipoproteins, we have shown the approach's unique ability to investigate key parameters affecting nanoparticle formation.

Original languageEnglish
Pages (from-to)2923-2926
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number11
StatePublished - 6 Mar 2017


  • FRET
  • block co-polymers
  • high-density lipoproteins
  • microfluidics
  • nanoemulsions


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