Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation

Remina Ikeda; Kyoko Ishii; Yoshiko Hoshikawa; Junya Azumi; Yuta Arakaki; Toshihiro Yasui; Shizuka Matsuura; Yoshiaki Matsumi; Yohei Kono; Yusuke Mizuta; Akihiro Kurimasa; Ichiro Hisatome; Scott L. Friedman; Hironaka Kawasaki; Goshi Shiota

doi:10.1007/s00011-011-0309-6

Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation

Remina Ikeda
, Kyoko Ishii
, Yoshiko Hoshikawa
, Junya Azumi
, Yuta Arakaki
, Toshihiro Yasui
, Shizuka Matsuura
, Yoshiaki Matsumi
, Yohei Kono
, Yusuke Mizuta
, Akihiro Kurimasa
, Ichiro Hisatome
, Scott L. Friedman
, Hironaka Kawasaki
, Goshi Shiota

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Objective and design: To clarify the molecular mechanism of polyenylphosphatidylcholine (PPC), we examined the involvement of reactive oxygen species (ROS) and NADPH oxidase 4 (Nox4) in human hepatic stellate cells (HSCs). Material: Using human LX-2 HSC cells, we examined the effects of PPC on expression of α-smooth muscle actin (α-SMA) and collagen 1, generation of ROS, Nox4 expression, p38 activation and cell proliferation, induced by transforming growth factor β1 (TGFβ1). Results: PPC suppressed ROS which are induced by TGFβ1, phosphorylation of p38MAPK, and expression levels of α-SMA and collagen 1 in a dose-dependent manner. Higher concentrations of PPC also suppressed Nox4 levels. Conclusion: These results suggest that ROS and Nox4 induced by TGFβ1 are the therapeutic targets of PPC in the suppression of human hepatic stellate cell activation.

Original language	English
Pages (from-to)	597-604
Number of pages	8
Journal	Inflammation Research
Volume	60
Issue number	6
DOIs	https://doi.org/10.1007/s00011-011-0309-6
State	Published - Jun 2011

Keywords

Hepatic stellate cells
NADPH oxidase 4
Oxidative stress
Polyenylphosphatidylcholine

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10.1007/s00011-011-0309-6

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Ikeda, R., Ishii, K., Hoshikawa, Y., Azumi, J., Arakaki, Y., Yasui, T., Matsuura, S., Matsumi, Y., Kono, Y., Mizuta, Y., Kurimasa, A., Hisatome, I., Friedman, S. L., Kawasaki, H., & Shiota, G. (2011). Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation. Inflammation Research, 60(6), 597-604. https://doi.org/10.1007/s00011-011-0309-6

@article{ed3db859d17c44baac12bf28ee61b32e,

title = "Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation",

abstract = "Objective and design: To clarify the molecular mechanism of polyenylphosphatidylcholine (PPC), we examined the involvement of reactive oxygen species (ROS) and NADPH oxidase 4 (Nox4) in human hepatic stellate cells (HSCs). Material: Using human LX-2 HSC cells, we examined the effects of PPC on expression of α-smooth muscle actin (α-SMA) and collagen 1, generation of ROS, Nox4 expression, p38 activation and cell proliferation, induced by transforming growth factor β1 (TGFβ1). Results: PPC suppressed ROS which are induced by TGFβ1, phosphorylation of p38MAPK, and expression levels of α-SMA and collagen 1 in a dose-dependent manner. Higher concentrations of PPC also suppressed Nox4 levels. Conclusion: These results suggest that ROS and Nox4 induced by TGFβ1 are the therapeutic targets of PPC in the suppression of human hepatic stellate cell activation.",

keywords = "Hepatic stellate cells, NADPH oxidase 4, Oxidative stress, Polyenylphosphatidylcholine",

author = "Remina Ikeda and Kyoko Ishii and Yoshiko Hoshikawa and Junya Azumi and Yuta Arakaki and Toshihiro Yasui and Shizuka Matsuura and Yoshiaki Matsumi and Yohei Kono and Yusuke Mizuta and Akihiro Kurimasa and Ichiro Hisatome and Friedman, \{Scott L.\} and Hironaka Kawasaki and Goshi Shiota",

year = "2011",

month = jun,

doi = "10.1007/s00011-011-0309-6",

language = "English",

volume = "60",

pages = "597--604",

journal = "Inflammation Research",

issn = "1023-3830",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "6",

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Ikeda, R, Ishii, K, Hoshikawa, Y, Azumi, J, Arakaki, Y, Yasui, T, Matsuura, S, Matsumi, Y, Kono, Y, Mizuta, Y, Kurimasa, A, Hisatome, I, Friedman, SL, Kawasaki, H & Shiota, G 2011, 'Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation', Inflammation Research, vol. 60, no. 6, pp. 597-604. https://doi.org/10.1007/s00011-011-0309-6

Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation. / Ikeda, Remina; Ishii, Kyoko; Hoshikawa, Yoshiko et al.
In: Inflammation Research, Vol. 60, No. 6, 06.2011, p. 597-604.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation

AU - Ikeda, Remina

AU - Ishii, Kyoko

AU - Hoshikawa, Yoshiko

AU - Azumi, Junya

AU - Arakaki, Yuta

AU - Yasui, Toshihiro

AU - Matsuura, Shizuka

AU - Matsumi, Yoshiaki

AU - Kono, Yohei

AU - Mizuta, Yusuke

AU - Kurimasa, Akihiro

AU - Hisatome, Ichiro

AU - Friedman, Scott L.

AU - Kawasaki, Hironaka

AU - Shiota, Goshi

PY - 2011/6

Y1 - 2011/6

N2 - Objective and design: To clarify the molecular mechanism of polyenylphosphatidylcholine (PPC), we examined the involvement of reactive oxygen species (ROS) and NADPH oxidase 4 (Nox4) in human hepatic stellate cells (HSCs). Material: Using human LX-2 HSC cells, we examined the effects of PPC on expression of α-smooth muscle actin (α-SMA) and collagen 1, generation of ROS, Nox4 expression, p38 activation and cell proliferation, induced by transforming growth factor β1 (TGFβ1). Results: PPC suppressed ROS which are induced by TGFβ1, phosphorylation of p38MAPK, and expression levels of α-SMA and collagen 1 in a dose-dependent manner. Higher concentrations of PPC also suppressed Nox4 levels. Conclusion: These results suggest that ROS and Nox4 induced by TGFβ1 are the therapeutic targets of PPC in the suppression of human hepatic stellate cell activation.

AB - Objective and design: To clarify the molecular mechanism of polyenylphosphatidylcholine (PPC), we examined the involvement of reactive oxygen species (ROS) and NADPH oxidase 4 (Nox4) in human hepatic stellate cells (HSCs). Material: Using human LX-2 HSC cells, we examined the effects of PPC on expression of α-smooth muscle actin (α-SMA) and collagen 1, generation of ROS, Nox4 expression, p38 activation and cell proliferation, induced by transforming growth factor β1 (TGFβ1). Results: PPC suppressed ROS which are induced by TGFβ1, phosphorylation of p38MAPK, and expression levels of α-SMA and collagen 1 in a dose-dependent manner. Higher concentrations of PPC also suppressed Nox4 levels. Conclusion: These results suggest that ROS and Nox4 induced by TGFβ1 are the therapeutic targets of PPC in the suppression of human hepatic stellate cell activation.

KW - Hepatic stellate cells

KW - NADPH oxidase 4

KW - Oxidative stress

KW - Polyenylphosphatidylcholine

UR - https://www.scopus.com/pages/publications/79956224825

U2 - 10.1007/s00011-011-0309-6

DO - 10.1007/s00011-011-0309-6

M3 - Article

C2 - 21318733

AN - SCOPUS:79956224825

SN - 1023-3830

VL - 60

SP - 597

EP - 604

JO - Inflammation Research

JF - Inflammation Research

IS - 6

ER -