TY - JOUR
T1 - Rapid, large-scale stimulated Raman histology with strip mosaicing and dual-phase detection
AU - Zhang, Bohan
AU - Sun, Mengxiong
AU - Yang, Yifan
AU - Chen, Lingchao
AU - Zou, Xiang
AU - Yang, Tian
AU - Hua, Yingqi
AU - Ji, Minbiao
N1 - Publisher Copyright:
© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Two-color stimulated Raman scattering (SRS) microscopy with label-free mapping of lipid/protein distributions has shown promise in virtual histology. Despite previous demonstrations of SRS in tumor delineation and diagnosis, the speed and efficiency of the current technique requires further improvements for practical use. Here, we integrate parallel dual-phase SRS detection with strip mosaicing, which reduces the imaging time of a whole mouse brain section from 70 to 8 minutes. We further verified our method in imaging fresh human surgical tissues, showing its great potential for rapid SRS histology, especially for large scale, large quantity imaging tasks.
AB - Two-color stimulated Raman scattering (SRS) microscopy with label-free mapping of lipid/protein distributions has shown promise in virtual histology. Despite previous demonstrations of SRS in tumor delineation and diagnosis, the speed and efficiency of the current technique requires further improvements for practical use. Here, we integrate parallel dual-phase SRS detection with strip mosaicing, which reduces the imaging time of a whole mouse brain section from 70 to 8 minutes. We further verified our method in imaging fresh human surgical tissues, showing its great potential for rapid SRS histology, especially for large scale, large quantity imaging tasks.
UR - http://www.scopus.com/inward/record.url?scp=85048190813&partnerID=8YFLogxK
U2 - 10.1364/BOE.9.002604
DO - 10.1364/BOE.9.002604
M3 - Article
AN - SCOPUS:85048190813
SN - 2156-7085
VL - 9
SP - 2604
EP - 2613
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 6
M1 - #325237
ER -