Rapid identification of chromosomal abnormalities in human neoplasia

A. Babu, R. S. Verma, H. Dosik

Research output: Contribution to journalArticlepeer-review


Recent advances in banding techniques have led to the belief that certain chromosomal defects are consistently associated with specific types of human neoplasia. Based on the GTG technique, it has been suggested that the malignant cells of most neoplasias show chromosomal abnormalities (Yunis et al. 1983). From this recent publication of Yunis it appears that the majority of bands involved in carcinogenesis are G-negative, i.e., do not stain by the GTG technique, and it is therefore difficult to localize the breakpoints. In some of our recent publications we emphasized the importance of the RFA technique (Verma and Lubs 1975), which stains Giemsa-negative bands darkly, thus providing precise identification of chromosomal abnormalities (Verma and Dosik 1976). However, this technique cannot be applied until the slides have aged for at least 7 days. Therefore, we are reporting an alternative procedure using BrdU which provides "reverse" banding immediately when the slides are stained with acridness orange and examined with a fluorescence microscope.

Original languageEnglish
Pages (from-to)321-323
Number of pages3
JournalBiotechnic and Histochemistry
Issue number5
StatePublished - 1986
Externally publishedYes


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