TY - JOUR
T1 - Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test
AU - D'Amato, R. F.
AU - Wallman, A. A.
AU - Hochstein, L. H.
AU - Colaninno, P. M.
AU - Scardamaglia, M.
AU - Ardila, E.
AU - Ghouri, M.
AU - Kim, K.
AU - Patel, R. C.
AU - Miller, A.
PY - 1995
Y1 - 1995
N2 - A rapid PCR-based test for the diagnosis of pulmonary tuberculosis, the Roche AMPLICOR Mycobacterium tuberculosis test (AMPLICOR MTB), was evaluated. Results from AMPLICOR MTB were compared with culture results and the final clinical diagnosis for each patient. A total of 985 specimens from 372 patients were tested. When AMPLICOR MTB results were compared with resolved results, i.e., a specimen grew M. tuberculosis or was obtained from a patient with a clinical diagnosis of tuberculosis, the sensitivity, specificity, positive predictive value, and negative predictive value for the AMPLICOR MTB test were 66.7, 99.6, 91.7, and 97.7%, respectively. These results were comparable to those obtained from culture. Test results were available approximately 6.5 h after specimen receipt in the laboratory. Our data demonstrate that AMPLICOR MTB will provide rapid, valuable information for the diagnosis and control of tuberculosis.
AB - A rapid PCR-based test for the diagnosis of pulmonary tuberculosis, the Roche AMPLICOR Mycobacterium tuberculosis test (AMPLICOR MTB), was evaluated. Results from AMPLICOR MTB were compared with culture results and the final clinical diagnosis for each patient. A total of 985 specimens from 372 patients were tested. When AMPLICOR MTB results were compared with resolved results, i.e., a specimen grew M. tuberculosis or was obtained from a patient with a clinical diagnosis of tuberculosis, the sensitivity, specificity, positive predictive value, and negative predictive value for the AMPLICOR MTB test were 66.7, 99.6, 91.7, and 97.7%, respectively. These results were comparable to those obtained from culture. Test results were available approximately 6.5 h after specimen receipt in the laboratory. Our data demonstrate that AMPLICOR MTB will provide rapid, valuable information for the diagnosis and control of tuberculosis.
UR - http://www.scopus.com/inward/record.url?scp=0029069284&partnerID=8YFLogxK
U2 - 10.1128/jcm.33.7.1832-1834.1995
DO - 10.1128/jcm.33.7.1832-1834.1995
M3 - Article
C2 - 7665654
AN - SCOPUS:0029069284
SN - 0095-1137
VL - 33
SP - 1832
EP - 1834
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 7
ER -