Rapid detection of mycoplasma-infected cell cultures

G. J. Todaro; S. A. Aaronson; Elaine Rands

doi:10.1016/S0014-4827(71)80077-0

Rapid detection of mycoplasma-infected cell cultures

G. J. Todaro, S. A. Aaronson, Elaine Rands

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

A method for the detection of mycoplasma-infected cell cultures is described which utilizes sucrose gradient sedimentation of supernatant fluids from cultures incubated with a radioactive precursor of RNA or DNA. The common strains of mycoplasma are found to band in sucrose at a density of 1.22-1.24 g/cm³.

Original language	English
Pages (from-to)	256-257
Number of pages	2
Journal	Experimental Cell Research
Volume	65
Issue number	1
DOIs	https://doi.org/10.1016/S0014-4827(71)80077-0
State	Published - Mar 1971
Externally published	Yes

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@article{ff55df7ba10942b1a6d9f0449511352e,

title = "Rapid detection of mycoplasma-infected cell cultures",

abstract = "A method for the detection of mycoplasma-infected cell cultures is described which utilizes sucrose gradient sedimentation of supernatant fluids from cultures incubated with a radioactive precursor of RNA or DNA. The common strains of mycoplasma are found to band in sucrose at a density of 1.22-1.24 g/cm3.",

author = "Todaro, {G. J.} and Aaronson, {S. A.} and Elaine Rands",

note = "Funding Information: This work was supported by NCI Contract No. PH 70-2074.",

year = "1971",

month = mar,

doi = "10.1016/S0014-4827(71)80077-0",

language = "English",

volume = "65",

pages = "256--257",

journal = "Experimental Cell Research",

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T1 - Rapid detection of mycoplasma-infected cell cultures

AU - Todaro, G. J.

AU - Aaronson, S. A.

AU - Rands, Elaine

N1 - Funding Information: This work was supported by NCI Contract No. PH 70-2074.

PY - 1971/3

Y1 - 1971/3

N2 - A method for the detection of mycoplasma-infected cell cultures is described which utilizes sucrose gradient sedimentation of supernatant fluids from cultures incubated with a radioactive precursor of RNA or DNA. The common strains of mycoplasma are found to band in sucrose at a density of 1.22-1.24 g/cm3.

AB - A method for the detection of mycoplasma-infected cell cultures is described which utilizes sucrose gradient sedimentation of supernatant fluids from cultures incubated with a radioactive precursor of RNA or DNA. The common strains of mycoplasma are found to band in sucrose at a density of 1.22-1.24 g/cm3.

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U2 - 10.1016/S0014-4827(71)80077-0

DO - 10.1016/S0014-4827(71)80077-0

M3 - Article

C2 - 4994336

AN - SCOPUS:0015027398

SN - 0014-4827

VL - 65

SP - 256

EP - 257

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 1

ER -

Rapid detection of mycoplasma-infected cell cultures

Abstract

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