Rapid and coordinated switch in chemokine receptor expression during dendritic cell maturation

Federica Sallusto, Patrick Schaerli, Pius Loetscher, Christoph Schaniel, Danielle Lenig, Charles R. Mackay, Shixin Qin, Antonio Lanzavecchia

Research output: Contribution to journalArticlepeer-review

953 Scopus citations


Dendritic cells (DC) migrate into inflamed peripheral tissues where they capture antigens and, following maturation, to lymph nodes where they stimulate T cells. To gain insight into this process we compared chemokine receptor expression in immature and mature DC. Immature DC expressed CCR1, CCR2, CCR5 and CXCR1 and responded to their respective ligands, which are chemokines produced at inflammatory sites. Following stimulation with LPS or TNF-α maturing DC expressed high levels of CCR7 mRNA and acquired responsiveness to the CCR7 ligand EBI1 ligand chemokine (ELC), a chemokine produced in lymphoid organs. Maturation also resulted in up-regulation of CXCR4 and down-regulation of CXCR1 mRNA, while CCR1 and CCR5 mRNA were only marginally affected for up to 40 h. However, CCR1 and CCR5 were lost from the cell surface within 3 h, due to receptor down-regulation mediated by chemokines produced by maturing DC. A complete down-regulation of CCR1 and CCR5 mRNA was observed only after stimulation with CD40 ligand of DC induced to mature by LPS treatment. These different patterns of chemokine receptors are consistent with 'inflammatory' and 'primary response' phases of DC function.

Original languageEnglish
Pages (from-to)2760-2769
Number of pages10
JournalEuropean Journal of Immunology
Issue number9
StatePublished - Sep 1998
Externally publishedYes


  • Chemokine receptor
  • Dendritic cell
  • Maturation


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