Radiosynthesis of 3-indolyl[1-11C]acetic acid for phyto-PET-imaging: An improved production procedure and formulation method

Youwen Xu; David L. Alexoff; Anna T. Kunert; Wenchao Qu; Dohyun Kim; Max Paven; Benjamin A. Babst; Richard A. Ferrieri; Michael J. Schueller; Joanna S. Fowler

doi:10.1016/j.apradiso.2014.05.017

Radiosynthesis of 3-indolyl[1-¹¹C]acetic acid for phyto-PET-imaging: An improved production procedure and formulation method

Youwen Xu
, David L. Alexoff
, Anna T. Kunert
, Wenchao Qu
, Dohyun Kim
, Max Paven
, Benjamin A. Babst
, Richard A. Ferrieri
, Michael J. Schueller
, Joanna S. Fowler

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

An improved production procedure and formulation method for the carbon-11 radiolabeled phytohormone, 3-indolyl-[l-¹¹C]acetic acid ([¹¹C]IAA), was developed by modifying selected original reaction parameters. This updated procedure both doubled the yield (from 25.9±6.7% (n=12) to 61.0±0.3% (n=10)) and increased the concentration (0.2-0.4GBq/0.15-0.3mL), enabling us to provide the radiotracer [¹¹C]IAA suitable for in vivo phyto-PET-imaging studies. The specific activity was improved by more than a factor of three (26.7±5.6GBq/μmol to 82.5±36.1GBq/μmol). The total synthesis time for both production and formulation was 81.8±3.0min (n=10). In addition, a streamlined semi-remote controlled production system, containing five processing modules, was designed and built for routine [¹¹C]IAA production. This integrated system facilitated routine high radiation level production of [¹¹C]IAA while minimizing radiation exposure to the production chemists.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Applied Radiation and Isotopes
Volume	91
DOIs	https://doi.org/10.1016/j.apradiso.2014.05.017
State	Published - Sep 2014
Externally published	Yes

Keywords

Formulation
Phyto-PET-imaging
Radiochemical yield
Specific activity

Access to Document

10.1016/j.apradiso.2014.05.017

Cite this

@article{ae72fbe72cc440d4b1122d33ae00c6a5,

title = "Radiosynthesis of 3-indolyl[1-11C]acetic acid for phyto-PET-imaging: An improved production procedure and formulation method",

abstract = "An improved production procedure and formulation method for the carbon-11 radiolabeled phytohormone, 3-indolyl-[l-11C]acetic acid ([11C]IAA), was developed by modifying selected original reaction parameters. This updated procedure both doubled the yield (from 25.9±6.7\% (n=12) to 61.0±0.3\% (n=10)) and increased the concentration (0.2-0.4GBq/0.15-0.3mL), enabling us to provide the radiotracer [11C]IAA suitable for in vivo phyto-PET-imaging studies. The specific activity was improved by more than a factor of three (26.7±5.6GBq/μmol to 82.5±36.1GBq/μmol). The total synthesis time for both production and formulation was 81.8±3.0min (n=10). In addition, a streamlined semi-remote controlled production system, containing five processing modules, was designed and built for routine [11C]IAA production. This integrated system facilitated routine high radiation level production of [11C]IAA while minimizing radiation exposure to the production chemists.",

keywords = "Formulation, Phyto-PET-imaging, Radiochemical yield, Specific activity",

author = "Youwen Xu and Alexoff, \{David L.\} and Kunert, \{Anna T.\} and Wenchao Qu and Dohyun Kim and Max Paven and Babst, \{Benjamin A.\} and Ferrieri, \{Richard A.\} and Schueller, \{Michael J.\} and Fowler, \{Joanna S.\}",

note = "Funding Information: This research was supported by the Office of Biological and Environmental Research , U. S. Department of Energy under Contract DE-AC02-98CH10886 . Additional support was provided by the German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) Bonn, which supported Anna T. Kunert and Max Paven. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States retains a non-exclusive, paid-up irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purpose. The authors thank Colleen Shea for editorial assistance. The authors also thanks to Colleen Shea, Lisa Muench, Carina Weber, and Sunny Kim for their helps in radiotracer production. ",

year = "2014",

month = sep,

doi = "10.1016/j.apradiso.2014.05.017",

language = "English",

volume = "91",

pages = "155--160",

journal = "Applied Radiation and Isotopes",

issn = "0969-8043",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Radiosynthesis of 3-indolyl[1-11C]acetic acid for phyto-PET-imaging

T2 - An improved production procedure and formulation method

AU - Xu, Youwen

AU - Alexoff, David L.

AU - Kunert, Anna T.

AU - Qu, Wenchao

AU - Kim, Dohyun

AU - Paven, Max

AU - Babst, Benjamin A.

AU - Ferrieri, Richard A.

AU - Schueller, Michael J.

AU - Fowler, Joanna S.

N1 - Funding Information: This research was supported by the Office of Biological and Environmental Research , U. S. Department of Energy under Contract DE-AC02-98CH10886 . Additional support was provided by the German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) Bonn, which supported Anna T. Kunert and Max Paven. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States retains a non-exclusive, paid-up irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purpose. The authors thank Colleen Shea for editorial assistance. The authors also thanks to Colleen Shea, Lisa Muench, Carina Weber, and Sunny Kim for their helps in radiotracer production.

PY - 2014/9

Y1 - 2014/9

N2 - An improved production procedure and formulation method for the carbon-11 radiolabeled phytohormone, 3-indolyl-[l-11C]acetic acid ([11C]IAA), was developed by modifying selected original reaction parameters. This updated procedure both doubled the yield (from 25.9±6.7% (n=12) to 61.0±0.3% (n=10)) and increased the concentration (0.2-0.4GBq/0.15-0.3mL), enabling us to provide the radiotracer [11C]IAA suitable for in vivo phyto-PET-imaging studies. The specific activity was improved by more than a factor of three (26.7±5.6GBq/μmol to 82.5±36.1GBq/μmol). The total synthesis time for both production and formulation was 81.8±3.0min (n=10). In addition, a streamlined semi-remote controlled production system, containing five processing modules, was designed and built for routine [11C]IAA production. This integrated system facilitated routine high radiation level production of [11C]IAA while minimizing radiation exposure to the production chemists.

AB - An improved production procedure and formulation method for the carbon-11 radiolabeled phytohormone, 3-indolyl-[l-11C]acetic acid ([11C]IAA), was developed by modifying selected original reaction parameters. This updated procedure both doubled the yield (from 25.9±6.7% (n=12) to 61.0±0.3% (n=10)) and increased the concentration (0.2-0.4GBq/0.15-0.3mL), enabling us to provide the radiotracer [11C]IAA suitable for in vivo phyto-PET-imaging studies. The specific activity was improved by more than a factor of three (26.7±5.6GBq/μmol to 82.5±36.1GBq/μmol). The total synthesis time for both production and formulation was 81.8±3.0min (n=10). In addition, a streamlined semi-remote controlled production system, containing five processing modules, was designed and built for routine [11C]IAA production. This integrated system facilitated routine high radiation level production of [11C]IAA while minimizing radiation exposure to the production chemists.

KW - Formulation

KW - Phyto-PET-imaging

KW - Radiochemical yield

KW - Specific activity

UR - https://www.scopus.com/pages/publications/84902489839

U2 - 10.1016/j.apradiso.2014.05.017

DO - 10.1016/j.apradiso.2014.05.017

M3 - Article

C2 - 24946093

AN - SCOPUS:84902489839

SN - 0969-8043

VL - 91

SP - 155

EP - 160

JO - Applied Radiation and Isotopes

JF - Applied Radiation and Isotopes