@article{913388dafee7452e8cd1ca58d1521aad,
title = "Radioiodine-labeled antibody elution (RIE) for detection of EBV-determined antigens: Competition for specific labeled antibodies using suspended antigens",
abstract = "Antigens determined by Epstein Barr virus (EBV) have been detected and quantitated in human lymphoblastoid cell culture lines by specific radioiodine-labeled antibody elution (RIE). The peak of specific elutable antibody has been shown to quantitatively increase with increasing doses of EBV 2-3 days after superinfection of susceptible lines. Increasing antibody concentrations on constant cell numbers produced a linear increase in elutable antibody binding specifically to the producer cells. Increasing cell numbers in the presence of a constant antibody concentration, likewise, produced a graded increase in the specific antibody eluted from the producer cells. Antigen suspensions prepared from frozen and thawed cells were used to complete with the antigens on the target cells for the antigen-combining sites of the labeled antibodies. Experiments were performed using suspensions from cells containing all the EBV-determined antigens for competition as well as limiting the competition to early antigen (EA).",
author = "Lamon, {E. W.} and F. Hilgers and A. Ros{\'e}n and G. Klein",
note = "Funding Information: {\textquoteright} These investigations were conducted under contract No. NO1 CP 3316 within the Virus Cancer Program of the National Cancer Institute, National Institutes of Health, Department of Health, Education, and Welfare; the Swedish Cancer Society, the Damon Runyon Memorial Fund,Ake Wiberg{\textquoteright}s Foundation, and Harald and Greta Jeansson{\textquoteright}s Foundation. The authors gratefully acknowledge the expert technical assistance of Miss Anitha Westman. * Recipient of NIH Training Grant ST01 GM01924-03, Clinical Investigators Present address: Department of Surgery, University of Alabama in Birmingham, Center, Birmingham, Alabama. z Supported by a collaborative research agreement with the International Agency for Research on Cancer, Lyon, France, and the Dutch Foundations, De Drie Lichten and the Josephus Jitta Fonds. Present address. The Netherlands Cancer Institute, Department of Biology, Sarphatis-traat 108 and the Laboratory for Medical Virology, University of Amsterdam, Mauritskade Amsterdam, Holland.",
year = "1974",
month = jul,
doi = "10.1016/0090-1229(74)90073-7",
language = "English",
volume = "2",
pages = "542--555",
journal = "Clinical Immunology and Immunopathology",
issn = "0090-1229",
publisher = "Academic Press Inc.",
number = "4",
}