Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with 111In-labeled new monoclonal antibody EBA-1 and F(ab′)2 fragments

Shrishailam Yemul; Jorge A. Leon; Ted Pozniakoff; Peter D. Esser; Alison Estabrook

doi:10.1016/0969-8051(93)90055-Y

Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with ¹¹¹In-labeled new monoclonal antibody EBA-1 and F(ab′)₂ fragments

Shrishailam Yemul, Jorge A. Leon, Ted Pozniakoff, Peter D. Esser, Alison Estabrook

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Radioimmunoimaging characteristics of a new monoclonal antibody EBA-1 and its F(ab′)₂ fragments utilizing nu/nu mice bearing human breast carcinoma xenografts are described. ¹¹¹In-DTPA conjugates of EBA-1 localized with tumor/blood ratios of 0.99 ± 0.10 (P < 0.3) and 4.65 ± 1.60 (P < 0.05), and localization indices of 1.25 and 2.61 at 24 and 72 h, respectively. A tumor/blood ratio as high as 28.86 ± 6.90 (P < 0.025) was obtained with EBA-1 F(ab′)₂ radioconjugates at 48 h. These results suggest that EBA-1 and its F(ab′)₂ might be useful reagents in radioimmunoimaging and radioimmunotherapy.

Original language	English
Pages (from-to)	325-335
Number of pages	11
Journal	Nuclear Medicine and Biology
Volume	20
Issue number	3
DOIs	https://doi.org/10.1016/0969-8051(93)90055-Y
State	Published - Apr 1993
Externally published	Yes

Access to Document

10.1016/0969-8051(93)90055-Y

Cite this

@article{89eba040a2964a8daa876fe5c75acc46,

title = "Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with 111In-labeled new monoclonal antibody EBA-1 and F(ab′)2 fragments",

abstract = "Radioimmunoimaging characteristics of a new monoclonal antibody EBA-1 and its F(ab′)2 fragments utilizing nu/nu mice bearing human breast carcinoma xenografts are described. 111In-DTPA conjugates of EBA-1 localized with tumor/blood ratios of 0.99 ± 0.10 (P < 0.3) and 4.65 ± 1.60 (P < 0.05), and localization indices of 1.25 and 2.61 at 24 and 72 h, respectively. A tumor/blood ratio as high as 28.86 ± 6.90 (P < 0.025) was obtained with EBA-1 F(ab′)2 radioconjugates at 48 h. These results suggest that EBA-1 and its F(ab′)2 might be useful reagents in radioimmunoimaging and radioimmunotherapy.",

author = "Shrishailam Yemul and Leon, {Jorge A.} and Ted Pozniakoff and Esser, {Peter D.} and Alison Estabrook",

note = "Funding Information: Acknowledgements-This research was supported by the Irving A. Hansen Memorial Foundation, and Florence and Herbert Irving Foundation. We thank Dr R. Mesa-Tejada for his encouragement during the course of this study.",

year = "1993",

month = apr,

doi = "10.1016/0969-8051(93)90055-Y",

language = "English",

volume = "20",

pages = "325--335",

journal = "Nuclear Medicine and Biology",

issn = "0969-8051",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with 111In-labeled new monoclonal antibody EBA-1 and F(ab′)2 fragments

AU - Yemul, Shrishailam

AU - Leon, Jorge A.

AU - Pozniakoff, Ted

AU - Esser, Peter D.

AU - Estabrook, Alison

N1 - Funding Information: Acknowledgements-This research was supported by the Irving A. Hansen Memorial Foundation, and Florence and Herbert Irving Foundation. We thank Dr R. Mesa-Tejada for his encouragement during the course of this study.

PY - 1993/4

Y1 - 1993/4

N2 - Radioimmunoimaging characteristics of a new monoclonal antibody EBA-1 and its F(ab′)2 fragments utilizing nu/nu mice bearing human breast carcinoma xenografts are described. 111In-DTPA conjugates of EBA-1 localized with tumor/blood ratios of 0.99 ± 0.10 (P < 0.3) and 4.65 ± 1.60 (P < 0.05), and localization indices of 1.25 and 2.61 at 24 and 72 h, respectively. A tumor/blood ratio as high as 28.86 ± 6.90 (P < 0.025) was obtained with EBA-1 F(ab′)2 radioconjugates at 48 h. These results suggest that EBA-1 and its F(ab′)2 might be useful reagents in radioimmunoimaging and radioimmunotherapy.

AB - Radioimmunoimaging characteristics of a new monoclonal antibody EBA-1 and its F(ab′)2 fragments utilizing nu/nu mice bearing human breast carcinoma xenografts are described. 111In-DTPA conjugates of EBA-1 localized with tumor/blood ratios of 0.99 ± 0.10 (P < 0.3) and 4.65 ± 1.60 (P < 0.05), and localization indices of 1.25 and 2.61 at 24 and 72 h, respectively. A tumor/blood ratio as high as 28.86 ± 6.90 (P < 0.025) was obtained with EBA-1 F(ab′)2 radioconjugates at 48 h. These results suggest that EBA-1 and its F(ab′)2 might be useful reagents in radioimmunoimaging and radioimmunotherapy.

UR - http://www.scopus.com/inward/record.url?scp=0027531005&partnerID=8YFLogxK

U2 - 10.1016/0969-8051(93)90055-Y

DO - 10.1016/0969-8051(93)90055-Y

M3 - Article

C2 - 8485492

AN - SCOPUS:0027531005

SN - 0969-8051

VL - 20

SP - 325

EP - 335

JO - Nuclear Medicine and Biology

JF - Nuclear Medicine and Biology

IS - 3

ER -

Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with 111In-labeled new monoclonal antibody EBA-1 and F(ab′)2 fragments

Abstract

Access to Document

Fingerprint

Cite this

Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with ¹¹¹In-labeled new monoclonal antibody EBA-1 and F(ab′)₂ fragments