Radioimmunoassay of leucine-enkephalin-like substance in human and canine plasma

Steven W. Ryder, John Eng

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Fasting human plasma immunoreactive leu-enkephalin (ir-leu-enkephalin) measured by radioimmunoassay averages 54±10 pg/ml. The method depends on acidification of plasma to protect against destruction of the peptide, adsorption to XAD-2 resin, extraction from the resin by aqueous methanol and concentration by evaporation. Plasma enkephalin in the dog increased from 13 to 56 pg/ml following insulin-induced hypoglycemia.

Original languageEnglish
Pages (from-to)367-369
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume52
Issue number2
DOIs
StatePublished - Feb 1981
Externally publishedYes

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