Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster resonance energy transfer

Torjus Skajaa; Yiming Zhao; Dave J. Van Den Heuvel; Hans C. Gerritsen; David P. Cormode; Rolf Koole; Matti M. Van Schooneveld; Jan Andries Post; Edward A. Fisher; Zahi A. Fayad; Celso De Mello Donega; Andries Meijerink; Willem J.M. Mulder

doi:10.1021/nl1037903

Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster resonance energy transfer

Torjus Skajaa, Yiming Zhao, Dave J. Van Den Heuvel, Hans C. Gerritsen, David P. Cormode, Rolf Koole, Matti M. Van Schooneveld, Jan Andries Post, Edward A. Fisher, Zahi A. Fayad, Celso De Mello Donega, Andries Meijerink, Willem J.M. Mulder

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67 Scopus citations

Abstract

The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Förster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein- lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.

Original language	English
Pages (from-to)	5131-5138
Number of pages	8
Journal	Nano Letters
Volume	10
Issue number	12
DOIs	https://doi.org/10.1021/nl1037903
State	Published - 8 Dec 2010

Keywords

Förster resonance energy transfer (FRET)
Quantum dots
apolipoprotein A-I
lipid-exchange
lipoproteins
live cell imaging

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10.1021/nl1037903

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Skajaa, T., Zhao, Y., Van Den Heuvel, D. J., Gerritsen, H. C., Cormode, D. P., Koole, R., Van Schooneveld, M. M., Post, J. A., Fisher, E. A., Fayad, Z. A., De Mello Donega, C., Meijerink, A., & Mulder, W. J. M. (2010). Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster resonance energy transfer. Nano Letters, 10(12), 5131-5138. https://doi.org/10.1021/nl1037903

@article{d006d3f2e35a498d9282065e5a634e2a,

title = "Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via F{\"o}rster resonance energy transfer",

abstract = "The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize F{\"o}rster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein- lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.",

keywords = "F{\"o}rster resonance energy transfer (FRET), Quantum dots, apolipoprotein A-I, lipid-exchange, lipoproteins, live cell imaging",

author = "Torjus Skajaa and Yiming Zhao and {Van Den Heuvel}, {Dave J.} and Gerritsen, {Hans C.} and Cormode, {David P.} and Rolf Koole and {Van Schooneveld}, {Matti M.} and Post, {Jan Andries} and Fisher, {Edward A.} and Fayad, {Zahi A.} and {De Mello Donega}, Celso and Andries Meijerink and Mulder, {Willem J.M.}",

year = "2010",

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day = "8",

doi = "10.1021/nl1037903",

language = "English",

volume = "10",

pages = "5131--5138",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

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Skajaa, T, Zhao, Y, Van Den Heuvel, DJ, Gerritsen, HC, Cormode, DP, Koole, R, Van Schooneveld, MM, Post, JA, Fisher, EA, Fayad, ZA, De Mello Donega, C, Meijerink, A & Mulder, WJM 2010, 'Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster resonance energy transfer', Nano Letters, vol. 10, no. 12, pp. 5131-5138. https://doi.org/10.1021/nl1037903

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AU - Skajaa, Torjus

AU - Zhao, Yiming

AU - Van Den Heuvel, Dave J.

AU - Gerritsen, Hans C.

AU - Cormode, David P.

AU - Koole, Rolf

AU - Van Schooneveld, Matti M.

AU - Post, Jan Andries

AU - Fisher, Edward A.

AU - Fayad, Zahi A.

AU - De Mello Donega, Celso

AU - Meijerink, Andries

AU - Mulder, Willem J.M.

PY - 2010/12/8

Y1 - 2010/12/8

N2 - The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Förster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein- lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.

AB - The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Förster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein- lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.

KW - Förster resonance energy transfer (FRET)

KW - Quantum dots

KW - apolipoprotein A-I

KW - lipid-exchange

KW - lipoproteins

KW - live cell imaging

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JO - Nano Letters

JF - Nano Letters

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ER -

Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster resonance energy transfer

Abstract

Keywords

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