@article{74e651a43580483c882310d48a438bab,
title = "Quantitative methods for the analysis of CFTR transcripts/splicing variants",
abstract = "In cystic fibrosis (CF), transcript analysis and quantification are important for diagnosis, prognosis and also as surrogate markers for some therapies including gene therapy. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods are evolving towards reliable quantification. Various protocols for the quantitative analysis of CFTR transcripts (including those resulting from splicing variants) are described and discussed here.",
keywords = "Alternative splicing, Quantitative PCR, RT-PCR, Real-time PCR, Splicing, Transcripts",
author = "Amaral, {Margarida D.} and Clarke, {Luka A.} and Ramalho, {Anabela S.} and Sebastian Beck and Fiona Broackes-Carter and Rebecca Rowntree and Nathalie Mouchel and Williams, {Sarah H.} and Ann Harris and Maria Tzetis and Bernhard Steiner and Javier Sanz and Sabina Gallati and Malka Nissim-Rafinifa and Batsheva Kerem and Timothy Hefferon and Cutting, {Garry R.} and Elisa Goina and Franco Pagani",
note = "Funding Information: The preparation of this review was supported by the EU-European Thematic Network on Cystic Fibrosis and Related Diseases (EU-QLK3-CT-1999-00241) and grants from the Italian Cystic Fibrosis Research Foundation, the Cystic Fibrosis Trust (UK), Vaincre La Mucoviscidose (France), the Portuguese Foundation for Science and Technology (POCTI/1999/MGI/35737; POCTI/MGI/47382/2002) and the EU (“CF-Chip” EU-QLK3-CT-2001-01982).",
year = "2004",
month = aug,
doi = "10.1016/j.jcf.2004.05.047",
language = "English",
volume = "3",
pages = "17--23",
journal = "Journal of Cystic Fibrosis",
issn = "1569-1993",
publisher = "Elsevier",
number = "SUPPL. 2",
}