Quantitative mass spectrometry identifies insulin signaling targets in C. elegans

Meng Qiu Dong; John D. Venable; Nora Au; Tao Xu; Kyu Park Sung; Daniel Cociorva; Jeffrey R. Johnson; Andrew Dillin; John R. Yates

doi:10.1126/science.1139952

Quantitative mass spectrometry identifies insulin signaling targets in C. elegans

Meng Qiu Dong, John D. Venable, Nora Au, Tao Xu, Kyu Park Sung, Daniel Cociorva, Jeffrey R. Johnson, Andrew Dillin, John R. Yates

Research output: Contribution to journal › Article › peer-review

272 Scopus citations

Abstract

DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.

Original language	English
Pages (from-to)	660-663
Number of pages	4
Journal	Science
Volume	317
Issue number	5838
DOIs	https://doi.org/10.1126/science.1139952
State	Published - 3 Aug 2007
Externally published	Yes

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abstract = "DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.",

author = "Dong, {Meng Qiu} and Venable, {John D.} and Nora Au and Tao Xu and Sung, {Kyu Park} and Daniel Cociorva and Johnson, {Jeffrey R.} and Andrew Dillin and Yates, {John R.}",

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AU - Venable, John D.

AU - Au, Nora

AU - Xu, Tao

AU - Sung, Kyu Park

AU - Cociorva, Daniel

AU - Johnson, Jeffrey R.

AU - Dillin, Andrew

AU - Yates, John R.

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AB - DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.

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Quantitative mass spectrometry identifies insulin signaling targets in C. elegans

Abstract

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