Quantitative mass spectrometry identifies insulin signaling targets in C. elegans

Meng Qiu Dong, John D. Venable, Nora Au, Tao Xu, Kyu Park Sung, Daniel Cociorva, Jeffrey R. Johnson, Andrew Dillin, John R. Yates

Research output: Contribution to journalArticlepeer-review

263 Scopus citations

Abstract

DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.

Original languageEnglish
Pages (from-to)660-663
Number of pages4
JournalScience
Volume317
Issue number5838
DOIs
StatePublished - 3 Aug 2007
Externally publishedYes

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