Quantitative determination of allotypic and idiotypic markers with antisera coupled to N hydroxysuccinimide activated Sepharose

A. B. Gottlieb, R. K. Seide, T. J. Kindt

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

N hydroxysuccinimide activated Sepharose beads (HAS) were used to immobilize allotypic and idiotypic antisera for use in radioimmunoassays. These HAS antisera were compared to antisera solidified with ethylchloroformate (ECF) with respect to their reactivity, specificity, and ability to detect subspecificities of the group a allotypes. The HAS antisera rated favorably in all comparisons with ECF antisera and are far superior with respect to ease of preparation and retention of antibody activity.

Original languageEnglish
Pages (from-to)51-54
Number of pages4
JournalJournal of Immunology
Volume114
Issue number1 I
StatePublished - 1975
Externally publishedYes

Fingerprint

Dive into the research topics of 'Quantitative determination of allotypic and idiotypic markers with antisera coupled to N hydroxysuccinimide activated Sepharose'. Together they form a unique fingerprint.

Cite this