Quantitative analysis of heparan sulfate using isotopically labeled calibrants

Zhangjie Wang; Katelyn Arnold; Yongmei Xu; Vijayakanth Pagadala; Guowei Su; Hannah Myatt; Robert J. Linhardt; Jian Liu

doi:10.1038/s42003-020-01150-6

Quantitative analysis of heparan sulfate using isotopically labeled calibrants

Zhangjie Wang, Katelyn Arnold, Yongmei Xu, Vijayakanth Pagadala, Guowei Su, Hannah Myatt, Robert J. Linhardt, Jian Liu

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19 Scopus citations

Abstract

Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight ¹³C-labeled disaccharide calibrants and one ¹³C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.

Original language	English
Article number	425
Journal	Communications Biology
Volume	3
Issue number	1
DOIs	https://doi.org/10.1038/s42003-020-01150-6
State	Published - 1 Dec 2020
Externally published	Yes

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abstract = "Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.",

author = "Zhangjie Wang and Katelyn Arnold and Yongmei Xu and Vijayakanth Pagadala and Guowei Su and Hannah Myatt and Linhardt, {Robert J.} and Jian Liu",

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AU - Arnold, Katelyn

AU - Xu, Yongmei

AU - Pagadala, Vijayakanth

AU - Su, Guowei

AU - Myatt, Hannah

AU - Linhardt, Robert J.

AU - Liu, Jian

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N2 - Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.

AB - Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.

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Quantitative analysis of heparan sulfate using isotopically labeled calibrants

Abstract

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