Quantitative analysis of flow microfluorometric data from asynchronous and drug-treated cell populations

Jerrold Fried; Xenophon Yataganas; Takeshi Kitahara; Amaury Perez; Ronald Ferguson; Susan Sullivan; Bayard Clarkson

doi:10.1016/0010-4809(76)90007-0

Quantitative analysis of flow microfluorometric data from asynchronous and drug-treated cell populations

Jerrold Fried
, Xenophon Yataganas
, Takeshi Kitahara
, Amaury Perez
, Ronald Ferguson
, Susan Sullivan
, Bayard Clarkson

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A new method for the mathematical analysis of data from flow microfluorometry is applied to data from cell populations growing exponentially or exposed to hydroxyurea or cyclic AMP. The analysis is not completely automatic, but requires interaction with the investigator. An algorithm for carrying out the analysis is described and illustrated with an example. Possible methods for simplifying the procedure are discussed. Studies with hydroxyurea-treated cells indicated that many cells were arrested in early S phase as well as in G₁. Some of the drug treated cells, especially those exposed for 3 to 4 days, had abnormally low fluorescence intensities. Cells treated with cyclic AMP for 72 hr accumulated mainly in the G₁ and G₂ phases, but some remained in S. Relatively pure fractions of G₁ cells (but not G₂) were obtained by cyclic AMP treatment followed by sucrose gradient fractionation.

Original language	English
Pages (from-to)	277-290
Number of pages	14
Journal	Computers and Biomedical Research
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/0010-4809(76)90007-0
State	Published - Jun 1976
Externally published	Yes

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@article{bd7b805c2ed44819a6ed938224661424,

title = "Quantitative analysis of flow microfluorometric data from asynchronous and drug-treated cell populations",

abstract = "A new method for the mathematical analysis of data from flow microfluorometry is applied to data from cell populations growing exponentially or exposed to hydroxyurea or cyclic AMP. The analysis is not completely automatic, but requires interaction with the investigator. An algorithm for carrying out the analysis is described and illustrated with an example. Possible methods for simplifying the procedure are discussed. Studies with hydroxyurea-treated cells indicated that many cells were arrested in early S phase as well as in G1. Some of the drug treated cells, especially those exposed for 3 to 4 days, had abnormally low fluorescence intensities. Cells treated with cyclic AMP for 72 hr accumulated mainly in the G1 and G2 phases, but some remained in S. Relatively pure fractions of G1 cells (but not G2) were obtained by cyclic AMP treatment followed by sucrose gradient fractionation.",

author = "Jerrold Fried and Xenophon Yataganas and Takeshi Kitahara and Amaury Perez and Ronald Ferguson and Susan Sullivan and Bayard Clarkson",

note = "Funding Information: This work was supported by grants from the National Cancer Institute, CA 08748 and CA 16757, the American Cancer Society, ACS-ET-2C and Cl-67A, and The Hearst Fund, The Lloyd Craver Fund. the United Leukemia Fund, The Grossinger Foundation Fund, and the Huntington Leukemia Research Fund.",

year = "1976",

month = jun,

doi = "10.1016/0010-4809(76)90007-0",

language = "English",

volume = "9",

pages = "277--290",

journal = "Computers and Biomedical Research",

issn = "0010-4809",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Quantitative analysis of flow microfluorometric data from asynchronous and drug-treated cell populations

AU - Fried, Jerrold

AU - Yataganas, Xenophon

AU - Kitahara, Takeshi

AU - Perez, Amaury

AU - Ferguson, Ronald

AU - Sullivan, Susan

AU - Clarkson, Bayard

N1 - Funding Information: This work was supported by grants from the National Cancer Institute, CA 08748 and CA 16757, the American Cancer Society, ACS-ET-2C and Cl-67A, and The Hearst Fund, The Lloyd Craver Fund. the United Leukemia Fund, The Grossinger Foundation Fund, and the Huntington Leukemia Research Fund.

PY - 1976/6

Y1 - 1976/6

N2 - A new method for the mathematical analysis of data from flow microfluorometry is applied to data from cell populations growing exponentially or exposed to hydroxyurea or cyclic AMP. The analysis is not completely automatic, but requires interaction with the investigator. An algorithm for carrying out the analysis is described and illustrated with an example. Possible methods for simplifying the procedure are discussed. Studies with hydroxyurea-treated cells indicated that many cells were arrested in early S phase as well as in G1. Some of the drug treated cells, especially those exposed for 3 to 4 days, had abnormally low fluorescence intensities. Cells treated with cyclic AMP for 72 hr accumulated mainly in the G1 and G2 phases, but some remained in S. Relatively pure fractions of G1 cells (but not G2) were obtained by cyclic AMP treatment followed by sucrose gradient fractionation.

AB - A new method for the mathematical analysis of data from flow microfluorometry is applied to data from cell populations growing exponentially or exposed to hydroxyurea or cyclic AMP. The analysis is not completely automatic, but requires interaction with the investigator. An algorithm for carrying out the analysis is described and illustrated with an example. Possible methods for simplifying the procedure are discussed. Studies with hydroxyurea-treated cells indicated that many cells were arrested in early S phase as well as in G1. Some of the drug treated cells, especially those exposed for 3 to 4 days, had abnormally low fluorescence intensities. Cells treated with cyclic AMP for 72 hr accumulated mainly in the G1 and G2 phases, but some remained in S. Relatively pure fractions of G1 cells (but not G2) were obtained by cyclic AMP treatment followed by sucrose gradient fractionation.

UR - https://www.scopus.com/pages/publications/0017078285

U2 - 10.1016/0010-4809(76)90007-0

DO - 10.1016/0010-4809(76)90007-0

M3 - Article

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AN - SCOPUS:0017078285

SN - 0010-4809

VL - 9

SP - 277

EP - 290

JO - Computers and Biomedical Research

JF - Computers and Biomedical Research

IS - 3

ER -

Quantitative analysis of flow microfluorometric data from asynchronous and drug-treated cell populations

Abstract

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