TY - JOUR
T1 - Quantitative analysis of flow microfluorometric data from asynchronous and drug-treated cell populations
AU - Fried, Jerrold
AU - Yataganas, Xenophon
AU - Kitahara, Takeshi
AU - Perez, Amaury
AU - Ferguson, Ronald
AU - Sullivan, Susan
AU - Clarkson, Bayard
N1 - Funding Information:
This work was supported by grants from the National Cancer Institute, CA 08748 and CA 16757, the American Cancer Society, ACS-ET-2C and Cl-67A, and The Hearst Fund, The Lloyd Craver Fund. the United Leukemia Fund, The Grossinger Foundation Fund, and the Huntington Leukemia Research Fund.
PY - 1976/6
Y1 - 1976/6
N2 - A new method for the mathematical analysis of data from flow microfluorometry is applied to data from cell populations growing exponentially or exposed to hydroxyurea or cyclic AMP. The analysis is not completely automatic, but requires interaction with the investigator. An algorithm for carrying out the analysis is described and illustrated with an example. Possible methods for simplifying the procedure are discussed. Studies with hydroxyurea-treated cells indicated that many cells were arrested in early S phase as well as in G1. Some of the drug treated cells, especially those exposed for 3 to 4 days, had abnormally low fluorescence intensities. Cells treated with cyclic AMP for 72 hr accumulated mainly in the G1 and G2 phases, but some remained in S. Relatively pure fractions of G1 cells (but not G2) were obtained by cyclic AMP treatment followed by sucrose gradient fractionation.
AB - A new method for the mathematical analysis of data from flow microfluorometry is applied to data from cell populations growing exponentially or exposed to hydroxyurea or cyclic AMP. The analysis is not completely automatic, but requires interaction with the investigator. An algorithm for carrying out the analysis is described and illustrated with an example. Possible methods for simplifying the procedure are discussed. Studies with hydroxyurea-treated cells indicated that many cells were arrested in early S phase as well as in G1. Some of the drug treated cells, especially those exposed for 3 to 4 days, had abnormally low fluorescence intensities. Cells treated with cyclic AMP for 72 hr accumulated mainly in the G1 and G2 phases, but some remained in S. Relatively pure fractions of G1 cells (but not G2) were obtained by cyclic AMP treatment followed by sucrose gradient fractionation.
UR - http://www.scopus.com/inward/record.url?scp=0017078285&partnerID=8YFLogxK
U2 - 10.1016/0010-4809(76)90007-0
DO - 10.1016/0010-4809(76)90007-0
M3 - Article
C2 - 181208
AN - SCOPUS:0017078285
SN - 0010-4809
VL - 9
SP - 277
EP - 290
JO - Computers and Biomedical Research
JF - Computers and Biomedical Research
IS - 3
ER -