TY - JOUR
T1 - Quantitation of preproenkephalin mRNA levels in brain regions from male Fischer rats following chronic cocaine treatment using a recently developed solution hybridization assay
AU - Branch, Andrea D.
AU - Unterwald, Ellen M.
AU - Lee, Susan E.
AU - Kreek, Mary Jeanne
N1 - Funding Information:
Acknowledgements. We wish to thank Dr. Charles E. Inturrisi and all the members of his laboratory, especially Drs. Steven O. Franklin and Yuan-Shan Zhu and Ms. Theresa Huang, for helping us to set up the quantitative solution hybridization systems. We are grateful to Ms. Jennifer Horne for assisting in all of the animal studies, to Dr. Ann Ho for sharing her insights into data analysis, to Dr. Inturrisi for his critical reading of the manuscript, and to Dr. Hugh D. Robertson for his support and encouragement. This work was supported in part by the NIDA Center Grant (DA-05130) and also in part by a grant from the Aaron Diamond Foundation. M.J.K. is the recipient of an ADAMHA-NIDA Research Scientist Award (DA-00040).
PY - 1992/7
Y1 - 1992/7
N2 - Quantitative solution hybridization assays were used to determine the picogram amounts of preproenkephalin mRNA (PPenk mRNA) and the microgram quantities of total rat RNA in extracts of eight brain regions from rats which had received three daily intraperitoneal injections of cocaine (10 or 30 mg/kg/day) or saline for 14 days. The young adult male Fischer rats were sacrificed 30 min after the final injection. The highest density of PPenk mRNA (pg PPenk mRNA/μg total cellular RNA) was found in extracts of striatum (34.08 ± 1.79 pg/μg for 11 saline-treated rats), followed by extracts of nucleus accumbens (10.08 ± 0.81 pg/μg), and extracts of hypothalamus (2.99 ± 0.31 pg/μg). Extracts of frontal cortex (1.78 ± 0.24 pg/μg), pituitary (1.39 ± 0.08 pg/μg), central grey (1.31 ± 0.16 pg/μg), and cerebellum (1.24 ± 0.09 pg/μg) had intermediate values. Extracts of hippocampus (0.53 ± 0.03 pg/μg) had the lowest density. No significant differences were found among the treatment groups in any brain area investigated. Therefore, chronic cocaine treatment as administered in this protocol did not alter expression of the gene encoding proenkephalin.
AB - Quantitative solution hybridization assays were used to determine the picogram amounts of preproenkephalin mRNA (PPenk mRNA) and the microgram quantities of total rat RNA in extracts of eight brain regions from rats which had received three daily intraperitoneal injections of cocaine (10 or 30 mg/kg/day) or saline for 14 days. The young adult male Fischer rats were sacrificed 30 min after the final injection. The highest density of PPenk mRNA (pg PPenk mRNA/μg total cellular RNA) was found in extracts of striatum (34.08 ± 1.79 pg/μg for 11 saline-treated rats), followed by extracts of nucleus accumbens (10.08 ± 0.81 pg/μg), and extracts of hypothalamus (2.99 ± 0.31 pg/μg). Extracts of frontal cortex (1.78 ± 0.24 pg/μg), pituitary (1.39 ± 0.08 pg/μg), central grey (1.31 ± 0.16 pg/μg), and cerebellum (1.24 ± 0.09 pg/μg) had intermediate values. Extracts of hippocampus (0.53 ± 0.03 pg/μg) had the lowest density. No significant differences were found among the treatment groups in any brain area investigated. Therefore, chronic cocaine treatment as administered in this protocol did not alter expression of the gene encoding proenkephalin.
KW - Central reward pathway
KW - Cocaine
KW - Preproenkephalin mRNA
KW - RNA quantitation
KW - Solution hybridization
UR - http://www.scopus.com/inward/record.url?scp=0026695315&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(92)90178-E
DO - 10.1016/0169-328X(92)90178-E
M3 - Article
C2 - 1279343
AN - SCOPUS:0026695315
SN - 0169-328X
VL - 14
SP - 231
EP - 238
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 3
ER -