Quantitation of Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) by a two-site enzyme immunoassay, in parallel with EBV-DNA

Lars Sternås; Lena Eliasson; Richard Lerner; George Klein

doi:10.1016/0022-1759(86)90352-2

Quantitation of Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) by a two-site enzyme immunoassay, in parallel with EBV-DNA

Lars Sternås, Lena Eliasson, Richard Lerner, George Klein

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A two-site enzyme (TSE) immunoassay was developed for the quantitation of the Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) using a rabbit serum raised against a synthetic peptide derived from the BamHI K region of the viral genome. Comparison of 12 EBNA-positive and 3 negative cell lines proved that the test was EBV-specific. A dot-blot assay utilizing cloned and nick translated EBV-DNA BamHI M fragment confirmed the EBV-carrier status of the EBNA-positive lines. The results obtained with both the TSE immunoassay and dot-blot assay were in agreement with published values. In contrast to earlier reports, we could not demonstrate any correlation between the content of EBNA and the number of viral genome copies.

Original language	English
Pages (from-to)	151-158
Number of pages	8
Journal	Journal of Immunological Methods
Volume	89
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(86)90352-2
State	Published - 22 May 1986
Externally published	Yes

Keywords

(EBNA)
(EBV)
EBV-DNA
ELISA for EBNA
Epstein-Barr virus
Epstein-Barr virus-determined nuclear antigen
Two-site enzyme immunoassay

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10.1016/0022-1759(86)90352-2

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title = "Quantitation of Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) by a two-site enzyme immunoassay, in parallel with EBV-DNA",

abstract = "A two-site enzyme (TSE) immunoassay was developed for the quantitation of the Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) using a rabbit serum raised against a synthetic peptide derived from the BamHI K region of the viral genome. Comparison of 12 EBNA-positive and 3 negative cell lines proved that the test was EBV-specific. A dot-blot assay utilizing cloned and nick translated EBV-DNA BamHI M fragment confirmed the EBV-carrier status of the EBNA-positive lines. The results obtained with both the TSE immunoassay and dot-blot assay were in agreement with published values. In contrast to earlier reports, we could not demonstrate any correlation between the content of EBNA and the number of viral genome copies.",

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doi = "10.1016/0022-1759(86)90352-2",

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AU - Sternås, Lars

AU - Eliasson, Lena

AU - Lerner, Richard

AU - Klein, George

PY - 1986/5/22

Y1 - 1986/5/22

N2 - A two-site enzyme (TSE) immunoassay was developed for the quantitation of the Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) using a rabbit serum raised against a synthetic peptide derived from the BamHI K region of the viral genome. Comparison of 12 EBNA-positive and 3 negative cell lines proved that the test was EBV-specific. A dot-blot assay utilizing cloned and nick translated EBV-DNA BamHI M fragment confirmed the EBV-carrier status of the EBNA-positive lines. The results obtained with both the TSE immunoassay and dot-blot assay were in agreement with published values. In contrast to earlier reports, we could not demonstrate any correlation between the content of EBNA and the number of viral genome copies.

AB - A two-site enzyme (TSE) immunoassay was developed for the quantitation of the Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) using a rabbit serum raised against a synthetic peptide derived from the BamHI K region of the viral genome. Comparison of 12 EBNA-positive and 3 negative cell lines proved that the test was EBV-specific. A dot-blot assay utilizing cloned and nick translated EBV-DNA BamHI M fragment confirmed the EBV-carrier status of the EBNA-positive lines. The results obtained with both the TSE immunoassay and dot-blot assay were in agreement with published values. In contrast to earlier reports, we could not demonstrate any correlation between the content of EBNA and the number of viral genome copies.

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KW - (EBV)

KW - EBV-DNA

KW - ELISA for EBNA

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Quantitation of Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) by a two-site enzyme immunoassay, in parallel with EBV-DNA

Abstract

Keywords

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