TY - JOUR
T1 - Quantitation of desmosine and isodesmosine in urine, plasma, and sputum by LC-MS/MS as biomarkers for elastin degradation
AU - Ma, Shuren
AU - Turino, Gerard M.
AU - Lin, Yong Y.
N1 - Funding Information:
This work was supported by funds from James P. Mara Center for Lung Diseases , the Flight Attendant Medical Research Institute , the Charles A Mastronardi Foundation , the Ned Doyle Foundation, the Alpha-1 Foundation , and by Funds from Ethel Kennedy, John Kennedy, and Judith Sulzberger .
PY - 2011/7/1
Y1 - 2011/7/1
N2 - The aim of this study is to develop a standardized LC-MS/MS method for accurate measurement of desmosine (DES) and isodesmosine (IDS) in all body fluids as biomarkers for in vivo degradation of matrix tissue elastin in man and animals. A reproducible three-step analytical procedure: (1) sample hydrolysis in 6. N HCl, (2) SPE by a CF1 cartridge with addition of acetylated pyridinoline as internal standard (IS), and (3) LC/MSMS analysis by SRM monitoring of transition ions; DES or IDS (m/. z 526-481. +. 397) and IS (m/. z 471-128) was developed. The method achieves accurate measurements of DES/IDS in accessible body fluids (i.e. urine, plasma, and sputum). LOQ of DES/IDS in body fluids is 0.1. ng/ml. The % recoveries and reproducibility from urine, plasma, and sputum samples are above 99 ± 8% (n= 3), 94 ± 9% n= 3) and 87 ± 11% (n= 3), with imprecision 8%, 9% and 10%, respectively. The proposed method was applied to measure DES/IDS in body fluids of patients with chronic obstructive pulmonary disease (COPD) and healthy controls. Total DES/IDS in sputum and plasma is increased over normal controls along with the free DES/IDS in urine in patients. DES/IDS can be used to study the course of COPD and the response to therapy. This practical and reliable LC-MS/MS method is proposed as a standardized method to measure DES and IDS in body fluids. This method can have wide application for investigating diseases which involve elastic tissue degradation.
AB - The aim of this study is to develop a standardized LC-MS/MS method for accurate measurement of desmosine (DES) and isodesmosine (IDS) in all body fluids as biomarkers for in vivo degradation of matrix tissue elastin in man and animals. A reproducible three-step analytical procedure: (1) sample hydrolysis in 6. N HCl, (2) SPE by a CF1 cartridge with addition of acetylated pyridinoline as internal standard (IS), and (3) LC/MSMS analysis by SRM monitoring of transition ions; DES or IDS (m/. z 526-481. +. 397) and IS (m/. z 471-128) was developed. The method achieves accurate measurements of DES/IDS in accessible body fluids (i.e. urine, plasma, and sputum). LOQ of DES/IDS in body fluids is 0.1. ng/ml. The % recoveries and reproducibility from urine, plasma, and sputum samples are above 99 ± 8% (n= 3), 94 ± 9% n= 3) and 87 ± 11% (n= 3), with imprecision 8%, 9% and 10%, respectively. The proposed method was applied to measure DES/IDS in body fluids of patients with chronic obstructive pulmonary disease (COPD) and healthy controls. Total DES/IDS in sputum and plasma is increased over normal controls along with the free DES/IDS in urine in patients. DES/IDS can be used to study the course of COPD and the response to therapy. This practical and reliable LC-MS/MS method is proposed as a standardized method to measure DES and IDS in body fluids. This method can have wide application for investigating diseases which involve elastic tissue degradation.
KW - Biomarker
KW - Chronic obstructive pulmonary disease
KW - Elastin degradation
KW - LC-MS
UR - http://www.scopus.com/inward/record.url?scp=80052958371&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2011.05.011
DO - 10.1016/j.jchromb.2011.05.011
M3 - Article
C2 - 21621489
AN - SCOPUS:80052958371
SN - 1570-0232
VL - 879
SP - 1893
EP - 1898
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 21
ER -