Quantification method for elemental bio-imaging by LA-ICP-MS using metal spiked PMMA films

Christine Austin; Dominic Hare; Tristan Rawling; Andrew M. McDonagh; Philip Doble

doi:10.1039/b911316a

Quantification method for elemental bio-imaging by LA-ICP-MS using metal spiked PMMA films

Christine Austin, Dominic Hare, Tristan Rawling, Andrew M. McDonagh, Philip Doble

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

A method for quantitative analysis of biological soft tissues by laser ablation-inductively coupled plasma-mass spectrometry has been developed. Polymer film standards were produced by spin coating spiked solutions of polymethylmethacrylate onto quartz substrates. Calibration curves throughout the range of 0-400 μg g^-1 yielded correlation coefficients better than 0.999 for ⁶⁶Zn and ⁶³Cu. Spiked, homogenised soft tissue standards were quantified by LA-ICP-MS against the thin film standards. The results agreed with values calculated from solution nebulisation ICP-MS. A procedure for internal standardisation by employing ruthenium or yttrium in the underlying thin film was also assessed.

Original language	English
Pages (from-to)	722-725
Number of pages	4
Journal	Journal of Analytical Atomic Spectrometry
Volume	25
Issue number	5
DOIs	https://doi.org/10.1039/b911316a
State	Published - 2010
Externally published	Yes

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title = "Quantification method for elemental bio-imaging by LA-ICP-MS using metal spiked PMMA films",

abstract = "A method for quantitative analysis of biological soft tissues by laser ablation-inductively coupled plasma-mass spectrometry has been developed. Polymer film standards were produced by spin coating spiked solutions of polymethylmethacrylate onto quartz substrates. Calibration curves throughout the range of 0-400 μg g-1 yielded correlation coefficients better than 0.999 for 66Zn and 63Cu. Spiked, homogenised soft tissue standards were quantified by LA-ICP-MS against the thin film standards. The results agreed with values calculated from solution nebulisation ICP-MS. A procedure for internal standardisation by employing ruthenium or yttrium in the underlying thin film was also assessed.",

author = "Christine Austin and Dominic Hare and Tristan Rawling and McDonagh, {Andrew M.} and Philip Doble",

year = "2010",

doi = "10.1039/b911316a",

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pages = "722--725",

journal = "Journal of Analytical Atomic Spectrometry",

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publisher = "Royal Society of Chemistry",

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T1 - Quantification method for elemental bio-imaging by LA-ICP-MS using metal spiked PMMA films

AU - Austin, Christine

AU - Hare, Dominic

AU - Rawling, Tristan

AU - McDonagh, Andrew M.

AU - Doble, Philip

PY - 2010

Y1 - 2010

N2 - A method for quantitative analysis of biological soft tissues by laser ablation-inductively coupled plasma-mass spectrometry has been developed. Polymer film standards were produced by spin coating spiked solutions of polymethylmethacrylate onto quartz substrates. Calibration curves throughout the range of 0-400 μg g-1 yielded correlation coefficients better than 0.999 for 66Zn and 63Cu. Spiked, homogenised soft tissue standards were quantified by LA-ICP-MS against the thin film standards. The results agreed with values calculated from solution nebulisation ICP-MS. A procedure for internal standardisation by employing ruthenium or yttrium in the underlying thin film was also assessed.

AB - A method for quantitative analysis of biological soft tissues by laser ablation-inductively coupled plasma-mass spectrometry has been developed. Polymer film standards were produced by spin coating spiked solutions of polymethylmethacrylate onto quartz substrates. Calibration curves throughout the range of 0-400 μg g-1 yielded correlation coefficients better than 0.999 for 66Zn and 63Cu. Spiked, homogenised soft tissue standards were quantified by LA-ICP-MS against the thin film standards. The results agreed with values calculated from solution nebulisation ICP-MS. A procedure for internal standardisation by employing ruthenium or yttrium in the underlying thin film was also assessed.

UR - http://www.scopus.com/inward/record.url?scp=77951840221&partnerID=8YFLogxK

U2 - 10.1039/b911316a

DO - 10.1039/b911316a

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VL - 25

SP - 722

EP - 725

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

IS - 5

ER -

Quantification method for elemental bio-imaging by LA-ICP-MS using metal spiked PMMA films

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