Qualitative study of fecal α1-proteinase inhibitor in normal subjects and patients with Crohn's disease

C. Mizon; C. Becuwe; M. Balduyck; J. F. Colombel; A. Cortot; J. Mizon; P. Degand

doi:10.1093/clinchem/34.11.2268

Qualitative study of fecal α₁-proteinase inhibitor in normal subjects and patients with Crohn's disease

C. Mizon, C. Becuwe, M. Balduyck, J. F. Colombel, A. Cortot, J. Mizon, P. Degand

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Abstract

We applied sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting to analyze fecal α₁-proteinase inhibitor (α₁PI) from healthy subjects and patient with Crohn's disease. A component with M(r) 38,000 was characterized in normal fecal extracts as well as in six pathological samples. In these cases, the Crohn's disease activity index (CDAI), a clinical index of severity of the disease, was 170 (SEM 47). In contrast, α₁PI of M(r) 51,000 was detected in fecal extracts from eight patients with active Crohn's disease (CDAI = 287, SEM 39). We conclude that fecal α₁PI can be considered a marker of intestinal disease activity.

Original language	English
Pages (from-to)	2268-2270
Number of pages	3
Journal	Clinical Chemistry
Volume	34
Issue number	11
DOIs	https://doi.org/10.1093/clinchem/34.11.2268
State	Published - 1988
Externally published	Yes

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title = "Qualitative study of fecal α1-proteinase inhibitor in normal subjects and patients with Crohn's disease",

abstract = "We applied sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting to analyze fecal α1-proteinase inhibitor (α1PI) from healthy subjects and patient with Crohn's disease. A component with M(r) 38,000 was characterized in normal fecal extracts as well as in six pathological samples. In these cases, the Crohn's disease activity index (CDAI), a clinical index of severity of the disease, was 170 (SEM 47). In contrast, α1PI of M(r) 51,000 was detected in fecal extracts from eight patients with active Crohn's disease (CDAI = 287, SEM 39). We conclude that fecal α1PI can be considered a marker of intestinal disease activity.",

author = "C. Mizon and C. Becuwe and M. Balduyck and Colombel, {J. F.} and A. Cortot and J. Mizon and P. Degand",

year = "1988",

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T1 - Qualitative study of fecal α1-proteinase inhibitor in normal subjects and patients with Crohn's disease

AU - Mizon, C.

AU - Becuwe, C.

AU - Balduyck, M.

AU - Colombel, J. F.

AU - Cortot, A.

AU - Mizon, J.

AU - Degand, P.

PY - 1988

Y1 - 1988

N2 - We applied sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting to analyze fecal α1-proteinase inhibitor (α1PI) from healthy subjects and patient with Crohn's disease. A component with M(r) 38,000 was characterized in normal fecal extracts as well as in six pathological samples. In these cases, the Crohn's disease activity index (CDAI), a clinical index of severity of the disease, was 170 (SEM 47). In contrast, α1PI of M(r) 51,000 was detected in fecal extracts from eight patients with active Crohn's disease (CDAI = 287, SEM 39). We conclude that fecal α1PI can be considered a marker of intestinal disease activity.

AB - We applied sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting to analyze fecal α1-proteinase inhibitor (α1PI) from healthy subjects and patient with Crohn's disease. A component with M(r) 38,000 was characterized in normal fecal extracts as well as in six pathological samples. In these cases, the Crohn's disease activity index (CDAI), a clinical index of severity of the disease, was 170 (SEM 47). In contrast, α1PI of M(r) 51,000 was detected in fecal extracts from eight patients with active Crohn's disease (CDAI = 287, SEM 39). We conclude that fecal α1PI can be considered a marker of intestinal disease activity.

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DO - 10.1093/clinchem/34.11.2268

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AN - SCOPUS:0024239260

SN - 0009-9147

VL - 34

SP - 2268

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JO - Clinical Chemistry

JF - Clinical Chemistry

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Qualitative study of fecal α1-proteinase inhibitor in normal subjects and patients with Crohn's disease

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Qualitative study of fecal α₁-proteinase inhibitor in normal subjects and patients with Crohn's disease