Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples

Xiaoqi Yang; Alisa Fox; Rebecca L.R. Powell

doi:10.1016/j.xpro.2022.101203

Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples

Xiaoqi Yang, Alisa Fox, Rebecca L.R. Powell

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer. For complete details on the use and execution of this protocol, please refer to Fox et al. (2020).

Original language	English
Article number	101203
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101203
State	Published - 18 Mar 2022

Keywords

Antibody
Health Sciences
Immunology
Protein Biochemistry

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10.1016/j.xpro.2022.101203

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title = "Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples",

abstract = "Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer. For complete details on the use and execution of this protocol, please refer to Fox et al. (2020).",

keywords = "Antibody, Health Sciences, Immunology, Protein Biochemistry",

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AU - Fox, Alisa

AU - Powell, Rebecca L.R.

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N2 - Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer. For complete details on the use and execution of this protocol, please refer to Fox et al. (2020).

AB - Antibodies in milk obtained from those previously SARS-CoV-2-infected or vaccinated against COVID-19 may provide passive immunity to the breastfed infant. Few assays have been established to measure antibodies in human milk, despite the public health importance of this topic. In the present protocol, we describe an optimized indirect ELISA assay aimed to measure SARS-CoV-2-reactive antibodies in human milk, which can be used as a rapid screen on undiluted samples or to designate samples as relatively low, moderate, or high titer. For complete details on the use and execution of this protocol, please refer to Fox et al. (2020).

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KW - Health Sciences

KW - Immunology

KW - Protein Biochemistry

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Qualitative immunoassay for the detection of anti-SARS-COV-2 spike antibody in human milk samples

Abstract

Keywords

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