Abstract
Dextran, a family of natural polysaccharides, consists of an α (1→6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to qualitative and quantitative analysis of dextran branches. A rotary cation-exchange cartridge array desalter was used for removal of salt from the HPAEC eluent making it MS compatible. MS and MS/MS were used to provide structural information on the enzymatically prepared dextran oligosaccharides. PAD provides quantitative data on the ratio of enzyme-resistant, branched dextran oligosaccharides. Both the types and degree of branching found in a variety of dextrans could be simultaneously determined online using this method.
Original language | English |
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Pages (from-to) | 79-85 |
Number of pages | 7 |
Journal | Journal of Chromatography A |
Volume | 1423 |
DOIs | |
State | Published - 4 Dec 2015 |
Externally published | Yes |
Keywords
- Branches
- Dextran
- HPAEC-MS
- Qualitative analysis
- Quantitative analysis