Qualitative and quantitative analysis of branches in dextran using high-performance anion exchange chromatography coupled to quadrupole time-of-flight mass spectrometry

Lin Yi, Yilan Ouyang, Xue Sun, Naiyu Xu, Robert J. Linhardt, Zhenqing Zhang

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Dextran, a family of natural polysaccharides, consists of an α (1→6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to qualitative and quantitative analysis of dextran branches. A rotary cation-exchange cartridge array desalter was used for removal of salt from the HPAEC eluent making it MS compatible. MS and MS/MS were used to provide structural information on the enzymatically prepared dextran oligosaccharides. PAD provides quantitative data on the ratio of enzyme-resistant, branched dextran oligosaccharides. Both the types and degree of branching found in a variety of dextrans could be simultaneously determined online using this method.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalJournal of Chromatography A
Volume1423
DOIs
StatePublished - 4 Dec 2015
Externally publishedYes

Keywords

  • Branches
  • Dextran
  • HPAEC-MS
  • Qualitative analysis
  • Quantitative analysis

Fingerprint

Dive into the research topics of 'Qualitative and quantitative analysis of branches in dextran using high-performance anion exchange chromatography coupled to quadrupole time-of-flight mass spectrometry'. Together they form a unique fingerprint.

Cite this