Pyrenemethyl laurate, a new fluorescent substrate for continuous kinetic determination of lipase activity

Anne Nègre, Robert Salvayre, Arie Dagan, Shimon Gatt

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A fluorescent acyl derivative of pyrenemethanol, pyrenemethyl laurate, was synthesized and used for the determination of several lipases by a continuous kinetic assay. The influence of the physical parameters of the substrate (pyrenemethyl laurate) and its hydrolysis product (pyrenemethanol), on the fluorescence emission was studied. The hydrolysis of pyrenemethyl laurate could be monitored directly in a spectrofluorometer because of the very high monomeric emission of pyrenemethanol at about 375 nm, whereas an aqueous dispersion of pyrenemethyl laurate emitted at 475 nm ('excimeric'). Pyrenemethyl laurate was hydrolyzed by gastric lipase, cellular lipases of haemopoietic cells, and the bacterial lipase of Rhizopus arrhizus.

Original languageEnglish
Pages (from-to)84-88
Number of pages5
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1006
Issue number1
DOIs
StatePublished - 6 Nov 1989
Externally publishedYes

Keywords

  • Fluorometry
  • Lipase activity
  • Pyrenemethanol
  • Pyrenemethyl laurate

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