Purification of neurocatin, a neuroregulatory factor from brain

Daniel K. Yee, Peter Pastuszko, Anna Pastuszko, David F. Wilson

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A neuroregulatory factor (neurocatin) has been isolated from bovine brain. Neurocatin is a powerful affector of catecholamine metabolism in synaptosomes isolated from rat brain, causing increased formation of norepinephrine (NE) and decreased formation of 3,4-dihydroxyphenylacetic acid (DOPAC). The ratio NE/DOPAC in synaptosomes has been used to measure the neurocatin content of samples during isolation. Neurocatin has now been purified to a single chromatographic peak by high-resolution HPLC and a preliminary amino acid content measured. It appears to be a small peptide with a molecular weight in the range of 2,000-2,500 Da.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalNeuroscience Letters
Volume114
Issue number2
DOIs
StatePublished - 3 Jul 1990
Externally publishedYes

Keywords

  • 3,4-Dihydroxyphenylacetic acid
  • Brain
  • Dopamine
  • Neuroregulatory factor
  • Norepinephrine
  • Synaptosome

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