Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa

Anqiang Zhang; Jiaying Deng; Shuying Yu; Fuming Zhang; Robert J. Linhardt; Peilong Sun

doi:10.1016/j.ijbiomac.2018.04.061

Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa

Anqiang Zhang, Jiaying Deng, Shuying Yu, Fuming Zhang, Robert J. Linhardt, Peilong Sun

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04 × 10⁶ Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography. Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (¹H NMR and ¹³C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra). GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑D‑Glcp‑(1 → [4)‑α‑D‑Glcp‑(1 → 4)‑α‑D‑Glcp‑(1]_m → 4)‑α‑D‑Glcp.

Original language	English
Pages (from-to)	221-226
Number of pages	6
Journal	International Journal of Biological Macromolecules
Volume	115
DOIs	https://doi.org/10.1016/j.ijbiomac.2018.04.061
State	Published - Aug 2018
Externally published	Yes

Keywords

Grifola frondosa
Polysaccharide
Purification
Structural elucidation

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10.1016/j.ijbiomac.2018.04.061

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@article{87a75c9dc9a345ae9f6b6994bcd7e25f,

title = "Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa",

abstract = "Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04 × 106 Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography. Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra). GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑D‑Glcp‑(1 → [4)‑α‑D‑Glcp‑(1 → 4)‑α‑D‑Glcp‑(1]m → 4)‑α‑D‑Glcp.",

keywords = "Grifola frondosa, Polysaccharide, Purification, Structural elucidation",

author = "Anqiang Zhang and Jiaying Deng and Shuying Yu and Fuming Zhang and Linhardt, \{Robert J.\} and Peilong Sun",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = aug,

doi = "10.1016/j.ijbiomac.2018.04.061",

language = "English",

volume = "115",

pages = "221--226",

journal = "International Journal of Biological Macromolecules",

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TY - JOUR

T1 - Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa

AU - Zhang, Anqiang

AU - Deng, Jiaying

AU - Yu, Shuying

AU - Zhang, Fuming

AU - Linhardt, Robert J.

AU - Sun, Peilong

PY - 2018/8

Y1 - 2018/8

N2 - Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04 × 106 Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography. Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra). GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑D‑Glcp‑(1 → [4)‑α‑D‑Glcp‑(1 → 4)‑α‑D‑Glcp‑(1]m → 4)‑α‑D‑Glcp.

AB - Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04 × 106 Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography. Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra). GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑D‑Glcp‑(1 → [4)‑α‑D‑Glcp‑(1 → 4)‑α‑D‑Glcp‑(1]m → 4)‑α‑D‑Glcp.

KW - Grifola frondosa

KW - Polysaccharide

KW - Purification

KW - Structural elucidation

UR - https://www.scopus.com/pages/publications/85045540891

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DO - 10.1016/j.ijbiomac.2018.04.061

M3 - Article

C2 - 29660454

AN - SCOPUS:85045540891

SN - 0141-8130

VL - 115

SP - 221

EP - 226

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa

Abstract

Keywords

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