Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS)

Chrystian Junqueira Alves; Alexandre Baldasserini Guimarães; Jessica Ruivo Maximino; Sergio Paulo Bydlowski; Gerson Chadi

doi:10.1007/978-1-4939-2313-7_11

Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS)

Chrystian Junqueira Alves
, Alexandre Baldasserini Guimarães
, Jessica Ruivo Maximino
, Sergio Paulo Bydlowski
, Gerson Chadi

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Olfactory ensheathing cells ( OECs ) are a specialized type of glia that supports axonal regeneration of olfactory neurons . OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders, like amyotrophic lateral sclerosis ( ALS ). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti-Thy 1.2 antibody leading to a specific cytotoxic lysis of contaminating fibroblasts without damaging the OECs. Purified OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors ( p75NTR ), are plated onto laminin-coated coverslips for immunofluorescence characterization, or Petri dishes for expansion and further investigations.

Original language	English
Title of host publication	Immunocytochemistry and Related Techniques
Publisher	Springer New York
Pages	195-208
Number of pages	14
ISBN (Electronic)	9781493923137
ISBN (Print)	9781493923120
DOIs	https://doi.org/10.1007/978-1-4939-2313-7_11
State	Published - 24 Feb 2015
Externally published	Yes

Keywords

Amyotrophic lateral sclerosis
Characterization
Mouse
Neurodegeneration
Olfactory ensheathing glia
Olfactory mucosa
Purification
Repair
Therapy

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10.1007/978-1-4939-2313-7_11

Cite this

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abstract = "Olfactory ensheathing cells ( OECs ) are a specialized type of glia that supports axonal regeneration of olfactory neurons . OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders, like amyotrophic lateral sclerosis ( ALS ). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti-Thy 1.2 antibody leading to a specific cytotoxic lysis of contaminating fibroblasts without damaging the OECs. Purified OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors ( p75NTR ), are plated onto laminin-coated coverslips for immunofluorescence characterization, or Petri dishes for expansion and further investigations.",

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Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS). / Alves, Chrystian Junqueira; Guimarães, Alexandre Baldasserini; Maximino, Jessica Ruivo et al.
Immunocytochemistry and Related Techniques. Springer New York, 2015. p. 195-208.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS)

AU - Alves, Chrystian Junqueira

AU - Guimarães, Alexandre Baldasserini

AU - Maximino, Jessica Ruivo

AU - Bydlowski, Sergio Paulo

AU - Chadi, Gerson

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015. All rights are reserved.

PY - 2015/2/24

Y1 - 2015/2/24

N2 - Olfactory ensheathing cells ( OECs ) are a specialized type of glia that supports axonal regeneration of olfactory neurons . OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders, like amyotrophic lateral sclerosis ( ALS ). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti-Thy 1.2 antibody leading to a specific cytotoxic lysis of contaminating fibroblasts without damaging the OECs. Purified OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors ( p75NTR ), are plated onto laminin-coated coverslips for immunofluorescence characterization, or Petri dishes for expansion and further investigations.

AB - Olfactory ensheathing cells ( OECs ) are a specialized type of glia that supports axonal regeneration of olfactory neurons . OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders, like amyotrophic lateral sclerosis ( ALS ). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti-Thy 1.2 antibody leading to a specific cytotoxic lysis of contaminating fibroblasts without damaging the OECs. Purified OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors ( p75NTR ), are plated onto laminin-coated coverslips for immunofluorescence characterization, or Petri dishes for expansion and further investigations.

KW - Amyotrophic lateral sclerosis

KW - Characterization

KW - Mouse

KW - Neurodegeneration

KW - Olfactory ensheathing glia

KW - Olfactory mucosa

KW - Purification

KW - Repair

KW - Therapy

UR - https://www.scopus.com/pages/publications/84954605461

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DO - 10.1007/978-1-4939-2313-7_11

M3 - Chapter

AN - SCOPUS:84954605461

SN - 9781493923120

SP - 195

EP - 208

BT - Immunocytochemistry and Related Techniques

PB - Springer New York

ER -

Purification and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS)

Abstract

Keywords

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Cite this