TY - JOUR
T1 - Purification and characterization of a cellular fibrinolytic factor associated with oncogenic transformation
T2 - The plasminogen activator from SV-40-transformed hamster cells
AU - Christman, Judith K.
AU - Acs, George
N1 - Funding Information:
Parler for their expert and dedicated assistance and Mr J.C. Unkeless and Dr E. Reich for discussion of their results prior to publication. These studies were supported by U.S.P.H.S. (CA-08751) and the American Cancer Society (NP-3601).
PY - 1974/3/27
Y1 - 1974/3/27
N2 - Neoplastic cells, whether transformed by oncogenic viruses or chemical agents, release a fibrinolytic factor not released by normal cells. We have purified this factor 14 000-fold from the supernatant culture fluid of SV-40-transformed hamster cells. It is a plasminogen activator with a molecular weight of approx. 50 000 consisting of subunits linked by disulphide bridges. It is irreversibly inhibited by diisopropylfluorophosphate, indicating that it is a serine protease. The subunit contianing the diisopropylfluorophosphate binding site has a molecular weight of approx. 25 000.
AB - Neoplastic cells, whether transformed by oncogenic viruses or chemical agents, release a fibrinolytic factor not released by normal cells. We have purified this factor 14 000-fold from the supernatant culture fluid of SV-40-transformed hamster cells. It is a plasminogen activator with a molecular weight of approx. 50 000 consisting of subunits linked by disulphide bridges. It is irreversibly inhibited by diisopropylfluorophosphate, indicating that it is a serine protease. The subunit contianing the diisopropylfluorophosphate binding site has a molecular weight of approx. 25 000.
UR - https://www.scopus.com/pages/publications/0015952549
U2 - 10.1016/0005-2787(74)90279-2
DO - 10.1016/0005-2787(74)90279-2
M3 - Article
C2 - 4363605
AN - SCOPUS:0015952549
SN - 0005-2787
VL - 340
SP - 339
EP - 347
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 3
ER -