Purifi cation and characterization of mouse olfactory ensheathing cells and their potential use for therapy in amyotrophic lateral sclerosis (ALS)

Chrystian Junqueira Alves, Alexandre Baldasserini Guimarães, Jessica Ruivo Maximino, Sergio Paulo Bydlowski, Gerson Chadi

Research output: Contribution to journalArticlepeer-review

Abstract

Olfactory ensheathing cells (OECs) are a specialized type of glia that supports axonal regeneration of olfactory neurons. OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders, like amyotrophic lateral sclerosis (ALS). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti- Thy 1.2 antibody leading to a specifi c cytotoxic lysis of contaminating fi broblasts without damaging the OECs. Purifi ed OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors (p75NTR), are plated onto laminin -coated coverslips for immunofl uorescence characterization, or Petri dishes for expansion and further investigations.

Original languageEnglish
Pages (from-to)195-208
Number of pages14
JournalNeuromethods
Volume101
DOIs
StatePublished - 2015

Keywords

  • Amyotrophic lateral sclerosis
  • Characterization
  • Mouse
  • Neurodegeneration
  • Olfactory ensheathing glia
  • Olfactory mucosa
  • Purifi cation
  • Repair
  • Therapy

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