Abstract
Olfactory ensheathing cells (OECs) are a specialized type of glia that supports axonal regeneration of olfactory neurons. OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders, like amyotrophic lateral sclerosis (ALS). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti- Thy 1.2 antibody leading to a specifi c cytotoxic lysis of contaminating fi broblasts without damaging the OECs. Purifi ed OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors (p75NTR), are plated onto laminin -coated coverslips for immunofl uorescence characterization, or Petri dishes for expansion and further investigations.
Original language | English |
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Pages (from-to) | 195-208 |
Number of pages | 14 |
Journal | Neuromethods |
Volume | 101 |
DOIs | |
State | Published - 2015 |
Keywords
- Amyotrophic lateral sclerosis
- Characterization
- Mouse
- Neurodegeneration
- Olfactory ensheathing glia
- Olfactory mucosa
- Purifi cation
- Repair
- Therapy