Pulse Cytophotometric Analysis of Synchronized Cells in Vitro

Pulse cytophotometry is a reliable rapid technique rendering a detailed direct analysis of the distribution of cells in G_1/0 S, and (G₂ + M) phase. We used a Phywe pulse cytophotometer ICP 11 to monitor cell cycle progression of synchronized human lymphoma cells in culture. With mithramycin as the fluorescent dye, sample processing is fast and provides DNA histograms of high resolution and precision. Results obtained from these histograms are in excellent agreement with those obtained by conventional techniques. Thus, we have established the conditions necessary to apply pulse cytophotometry for studies of drug-induced cytokinetic effects on this cell line.