Proton magnetic resonance studies at 220 MHz of the histidine residues of staphylococcal nuclease

Jack S. Cohen, Richard I. Shrager, Mildred Mcneel, Alan N. Schechter

Research output: Contribution to journalShort surveypeer-review

43 Scopus citations

Abstract

The imidazole C2-H resonances in the 220 MHz proton magnetic resonance spectra of staphylococcal nuclease have been identified with the aid of computer curve-fitting. Spectra were fitted to Lorentzian curves for the measurement of peak area, and chemical shifts were fitted to theoretical pH titration curves for the precise determination of pK values. Such a high resolution analysis makes a conformational equilibrium hypothesis involving histidine unlikely in the case of nuclease.

Original languageEnglish
Pages (from-to)642-644
Number of pages3
JournalNature
Volume228
Issue number5272
DOIs
StatePublished - 1970
Externally publishedYes

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