Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids

Anne Zebitz Eriksen; Rasmus Møller; Bar Makovoz; Benjamin R. tenOever; Timothy A. Blenkinsop

doi:10.1016/j.xpro.2022.101383

Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids

Anne Zebitz Eriksen, Rasmus Møller, Bar Makovoz, Benjamin R. tenOever, Timothy A. Blenkinsop

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).

Original language	English
Article number	101383
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101383
State	Published - 17 Jun 2022

Keywords

Health Sciences
Immunology
Microbiology
Organoids
Stem Cells

Access to Document

10.1016/j.xpro.2022.101383

Cite this

@article{cd65fe2339c64031b2bbba19e77d9687,

title = "Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids",

abstract = "Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).",

keywords = "Health Sciences, Immunology, Microbiology, Organoids, Stem Cells",

author = "Eriksen, {Anne Zebitz} and Rasmus M{\o}ller and Bar Makovoz and tenOever, {Benjamin R.} and Blenkinsop, {Timothy A.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = jun,

day = "17",

doi = "10.1016/j.xpro.2022.101383",

language = "English",

volume = "3",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids

AU - Eriksen, Anne Zebitz

AU - Møller, Rasmus

AU - Makovoz, Bar

AU - tenOever, Benjamin R.

AU - Blenkinsop, Timothy A.

PY - 2022/6/17

Y1 - 2022/6/17

N2 - Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).

AB - Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).

KW - Health Sciences

KW - Immunology

KW - Microbiology

KW - Organoids

KW - Stem Cells

UR - http://www.scopus.com/inward/record.url?scp=85131265973&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2022.101383

DO - 10.1016/j.xpro.2022.101383

M3 - Article

C2 - 35664254

AN - SCOPUS:85131265973

SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 101383

ER -

Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids

Abstract

Keywords

Access to Document

Fingerprint

Cite this