Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids

Anne Zebitz Eriksen, Rasmus Møller, Bar Makovoz, Benjamin R. tenOever, Timothy A. Blenkinsop

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).

Original languageEnglish
Article number101383
JournalSTAR Protocols
Issue number2
StatePublished - 17 Jun 2022


  • Health Sciences
  • Immunology
  • Microbiology
  • Organoids
  • Stem Cells


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