@article{cd65fe2339c64031b2bbba19e77d9687,
title = "Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids",
abstract = "Here, we describe a series of protocols detailing the steps for evaluating SARS-CoV-2 infection in models of the human eye. Included are protocols for whole eye organoid differentiation, SARS-CoV-2 infection, and processing organoids for single-cell RNA sequencing. Additional protocols describe how to dissect and culture adult human ocular cells from cadaver donor eyes and how to compare infection of SARS-CoV-2 and the presence of SARS-CoV-2 entry factors using qPCR, immunofluorescence, and plaque assays. For complete details on the use and execution of this protocol, please refer to Eriksen et al. (2021).",
keywords = "Health Sciences, Immunology, Microbiology, Organoids, Stem Cells",
author = "Eriksen, {Anne Zebitz} and Rasmus M{\o}ller and Bar Makovoz and tenOever, {Benjamin R.} and Blenkinsop, {Timothy A.}",
note = "Funding Information: We would like to extend our gratitude to the patients and their families for their generous donations, as well as the Eye Bank for Sight Restoration for their support and work with processing donations. We would like to thank the Lundbeck Foundation, R322-2019-2611, the Carlsberg Foundation, a Challenge Grant award from Research to Prevent Blindness, the National Eye Institute (NEI), and extramural grants 1R21EY030215-01, 1R01EY029736-01A1 and Icahn School of Medicine at Mount Sinai for supporting this work. Conceptualization, T.A.B. and B.R.t.; Methodology, R.M. B.M. and A.Z.E.; Resources, T.A.B.; Investigation, R.M. B.M. and A.Z.E.; Visualization, R.M. and A.Z.E.; Writing, A.Z.E. and R.M.; Editing, T.A.B.; Supervision, T.A.B. and B.R.t.; Funding, T.B.A. and B.R.t.; Project administration, T.A.B. and B.R.t. The authors declare no competing interests. Funding Information: We would like to extend our gratitude to the patients and their families for their generous donations, as well as the Eye Bank for Sight Restoration for their support and work with processing donations. We would like to thank the Lundbeck Foundation , R322-2019-2611 , the Carlsberg Foundation , a Challenge Grant award from Research to Prevent Blindness, the National Eye Institute (NEI), and extramural grants 1R21EY030215-01 , 1R01EY029736-01A1 and Icahn School of Medicine at Mount Sinai for supporting this work. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
month = jun,
day = "17",
doi = "10.1016/j.xpro.2022.101383",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}