Protocol for optimizing production and quality control of infective EcoHIV virions

Hammodah R. Alfar, Daphne N. Pariser, Harry Chanzu, Smita Joshi, Daniëlle M. Coenen, Joshua Lykins, Kanakanagavalli Shravani Prakhya, Mary Jane Potash, Wei Chao, Jennifer Kelschenbach, David J. Volsky, Kelly Metcalf-Pate, Sidney W. Whiteheart

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

Original languageEnglish
Article number102368
JournalSTAR Protocols
Volume4
Issue number3
DOIs
StatePublished - 15 Sep 2023

Keywords

  • Cell Culture
  • Cell-based Assays
  • Health Sciences

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