Protocol for optimizing production and quality control of infective EcoHIV virions

Hammodah R. Alfar; Daphne N. Pariser; Harry Chanzu; Smita Joshi; Daniëlle M. Coenen; Joshua Lykins; Kanakanagavalli Shravani Prakhya; Mary Jane Potash; Wei Chao; Jennifer Kelschenbach; David J. Volsky; Kelly Metcalf-Pate; Sidney W. Whiteheart

doi:10.1016/j.xpro.2023.102368

Protocol for optimizing production and quality control of infective EcoHIV virions

Hammodah R. Alfar, Daphne N. Pariser, Harry Chanzu, Smita Joshi, Daniëlle M. Coenen, Joshua Lykins, Kanakanagavalli Shravani Prakhya, Mary Jane Potash, Wei Chao, Jennifer Kelschenbach, David J. Volsky, Kelly Metcalf-Pate, Sidney W. Whiteheart

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

Original language	English
Article number	102368
Journal	STAR Protocols
Volume	4
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2023.102368
State	Published - 15 Sep 2023

Keywords

Cell Culture
Cell-based Assays
Health Sciences

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title = "Protocol for optimizing production and quality control of infective EcoHIV virions",

abstract = "EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.",

keywords = "Cell Culture, Cell-based Assays, Health Sciences",

author = "Alfar, {Hammodah R.} and Pariser, {Daphne N.} and Harry Chanzu and Smita Joshi and Coenen, {Dani{\"e}lle M.} and Joshua Lykins and Prakhya, {Kanakanagavalli Shravani} and Potash, {Mary Jane} and Wei Chao and Jennifer Kelschenbach and Volsky, {David J.} and Kelly Metcalf-Pate and Whiteheart, {Sidney W.}",

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doi = "10.1016/j.xpro.2023.102368",

language = "English",

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TY - JOUR

T1 - Protocol for optimizing production and quality control of infective EcoHIV virions

AU - Alfar, Hammodah R.

AU - Pariser, Daphne N.

AU - Chanzu, Harry

AU - Joshi, Smita

AU - Coenen, Daniëlle M.

AU - Lykins, Joshua

AU - Prakhya, Kanakanagavalli Shravani

AU - Potash, Mary Jane

AU - Chao, Wei

AU - Kelschenbach, Jennifer

AU - Volsky, David J.

AU - Metcalf-Pate, Kelly

AU - Whiteheart, Sidney W.

PY - 2023/9/15

Y1 - 2023/9/15

N2 - EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

AB - EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

KW - Cell Culture

KW - Cell-based Assays

KW - Health Sciences

UR - http://www.scopus.com/inward/record.url?scp=85162181690&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2023.102368

DO - 10.1016/j.xpro.2023.102368

M3 - Article

AN - SCOPUS:85162181690

SN - 2666-1667

VL - 4

JO - STAR Protocols

JF - STAR Protocols

IS - 3

M1 - 102368

ER -

Protocol for optimizing production and quality control of infective EcoHIV virions

Abstract

Keywords

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