Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

Xinzheng Guo; Christopher Starr; Jing Zhou; Bo Chen

doi:10.1016/j.xpro.2021.100932

Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

Xinzheng Guo, Christopher Starr, Jing Zhou, Bo Chen

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).

Original language	English
Article number	100932
Journal	STAR Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2021.100932
State	Published - 17 Dec 2021

Keywords

Cell Biology
Cell-based Assays
Gene Expression
Microscopy
Model Organisms
Neuroscience

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10.1016/j.xpro.2021.100932

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abstract = "The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).",

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Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

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Keywords

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