TY - JOUR
T1 - Proteomic characterization of atopic dermatitis blood from infancy to adulthood
AU - Del Duca, Ester
AU - Renert-Yuval, Yael
AU - Pavel, Ana B.
AU - Mikhaylov, Daniela
AU - Wu, Jianni
AU - Lefferdink, Rachel
AU - Fang, Milie
AU - Sheth, Anjani
AU - Blumstein, Alli
AU - Facheris, Paola
AU - Estrada, Yeriel D.
AU - Rangel, Stephanie M.
AU - Krueger, James G.
AU - Paller, Amy S.
AU - Guttman-Yassky, Emma
N1 - Publisher Copyright:
© 2023 American Academy of Dermatology, Inc.
PY - 2023/5
Y1 - 2023/5
N2 - Background: Patients with atopic dermatitis (AD) have systemic biomarker dysregulation that differs by age group; however, the proteomic characteristics of these age-based changes are unknown. Objective: To profile blood proteins of patients with AD across different age groups versus age-appropriate controls. Methods: Using the Olink high-throughput proteomic platform, we profiled 375 serum proteins of 20 infants (age, 0-5 years), 39 children (age, 6-11 years), 21 adolescents (age, 12-17 years), and 20 adults (age, ≥18 years) with moderate-to-severe AD and 83 age-appropriate controls. Results: Each group presented a distinct systemic proteomic signature. Th2-related proteins were increased in infant AD and further intensified with age through adolescence and adulthood (interleukin 4/CCL13/CCL17). In contrast, Th1 axis down-regulation was detected in infants with AD and gradually reversed to increased Th1 products (interferon γ/CXCL9/CXCL10/CCL2) in patients with AD from childhood to adulthood. Despite their short disease duration, infants already had evidence of systemic inflammation, with significant upregulation of innate immunity (interleukin 17C/ interleukin-1RN), T-cell activation/migration (CCL19), Th2 (CCL13/CCL17), and Th17 (PI3) proteins. Adults with AD present unique upregulation of cardiovascular proteins related to coagulation and diabetes. Limitations: Cross-sectional observational study with a single time point. Conclusion: Systemic immune signatures of AD are age-specific beyond the shared Th2 immune activation. These data advocate for precision medicine approaches based on age-specific AD profiles.
AB - Background: Patients with atopic dermatitis (AD) have systemic biomarker dysregulation that differs by age group; however, the proteomic characteristics of these age-based changes are unknown. Objective: To profile blood proteins of patients with AD across different age groups versus age-appropriate controls. Methods: Using the Olink high-throughput proteomic platform, we profiled 375 serum proteins of 20 infants (age, 0-5 years), 39 children (age, 6-11 years), 21 adolescents (age, 12-17 years), and 20 adults (age, ≥18 years) with moderate-to-severe AD and 83 age-appropriate controls. Results: Each group presented a distinct systemic proteomic signature. Th2-related proteins were increased in infant AD and further intensified with age through adolescence and adulthood (interleukin 4/CCL13/CCL17). In contrast, Th1 axis down-regulation was detected in infants with AD and gradually reversed to increased Th1 products (interferon γ/CXCL9/CXCL10/CCL2) in patients with AD from childhood to adulthood. Despite their short disease duration, infants already had evidence of systemic inflammation, with significant upregulation of innate immunity (interleukin 17C/ interleukin-1RN), T-cell activation/migration (CCL19), Th2 (CCL13/CCL17), and Th17 (PI3) proteins. Adults with AD present unique upregulation of cardiovascular proteins related to coagulation and diabetes. Limitations: Cross-sectional observational study with a single time point. Conclusion: Systemic immune signatures of AD are age-specific beyond the shared Th2 immune activation. These data advocate for precision medicine approaches based on age-specific AD profiles.
KW - Olink
KW - atopic dermatitis
KW - pediatric
KW - proteomic
UR - http://www.scopus.com/inward/record.url?scp=85149675222&partnerID=8YFLogxK
U2 - 10.1016/j.jaad.2022.12.050
DO - 10.1016/j.jaad.2022.12.050
M3 - Article
C2 - 36773824
AN - SCOPUS:85149675222
SN - 0190-9622
VL - 88
SP - 1083
EP - 1093
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
IS - 5
ER -