TY - JOUR
T1 - Proteomic analysis reveals differentially expressed proteins in the rat frontal cortex after methamphetamine treatment
AU - Faure, J. J.
AU - Hattingh, S. M.
AU - Stein, D. J.
AU - Daniels, W. M.
N1 - Funding Information:
Acknowledgements The authors would like to acknowledge the contributions of the Biological Mass Spectrometry and Proteomics Facility in the Department of Biological Sciences, University of Warwick. This work was supported by a grant from the Medical Research Council (MRC).
PY - 2009/12
Y1 - 2009/12
N2 - Methamphetamine (MA) is an addictive psycho-stimulant and the illicit use of the drug is escalating. In the present study, we examined protein expression profiles in the rat frontal cortex exposed to a total of eight MA injections (1 mg/kg, intraperitoneal) using 2-DE based proteomics. We investigated protein changes occurring in both the cytosolic fraction and the membrane fraction. 2-DE analysis resulted in 62 cytosolic and 44 membrane protein spots that were differentially regulated in the frontal cortex of rats exposed to MA when compared to control animals. Of these spots, 47 cytosolic and 42 membrane proteins were identified respectively, using ESI-Quad-TOF, which included ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), β-synuclein, 78 kDa glucose-regulated protein (GRP 78), γ-enolase, dihydropyrimidase- related protein 2 (DRP 2), complexin 2 and synapsin II. These proteins are associated with protein degradation, redox regulation, energy metabolism, cellular growth, cytoskeletal modifications and synaptic function. Proteomic research may be useful in exploring the complex underlying molecular mechanisms of MA dependence.
AB - Methamphetamine (MA) is an addictive psycho-stimulant and the illicit use of the drug is escalating. In the present study, we examined protein expression profiles in the rat frontal cortex exposed to a total of eight MA injections (1 mg/kg, intraperitoneal) using 2-DE based proteomics. We investigated protein changes occurring in both the cytosolic fraction and the membrane fraction. 2-DE analysis resulted in 62 cytosolic and 44 membrane protein spots that were differentially regulated in the frontal cortex of rats exposed to MA when compared to control animals. Of these spots, 47 cytosolic and 42 membrane proteins were identified respectively, using ESI-Quad-TOF, which included ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), β-synuclein, 78 kDa glucose-regulated protein (GRP 78), γ-enolase, dihydropyrimidase- related protein 2 (DRP 2), complexin 2 and synapsin II. These proteins are associated with protein degradation, redox regulation, energy metabolism, cellular growth, cytoskeletal modifications and synaptic function. Proteomic research may be useful in exploring the complex underlying molecular mechanisms of MA dependence.
KW - Cytosolic proteins
KW - Frontal cortex
KW - Membrane proteins
KW - Methamphetamine
KW - Neuronal plasticity
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=74449084485&partnerID=8YFLogxK
U2 - 10.1007/s11011-009-9167-0
DO - 10.1007/s11011-009-9167-0
M3 - Article
C2 - 19826936
AN - SCOPUS:74449084485
SN - 0885-7490
VL - 24
SP - 685
EP - 700
JO - Metabolic Brain Disease
JF - Metabolic Brain Disease
IS - 4
ER -