Proteomic analysis of sera from hepatocellular carcinoma patients after radiofrequency ablation treatment

Takayuki Kawakami, Yujin Hoshida, Fumihiko Kanai, Yasuo Tanaka, Keisuke Tateishi, Tsuneo Ikenoue, Shuntaro Obi, Shinpei Sato, Takuma Teratani, Shuichiro Shiina, Takao Kawabe, Takamasa Suzuki, Naoya Hatano, Hisaaki Taniguchi, Masao Omata

Research output: Contribution to journalArticlepeer-review

57 Scopus citations


Comparative proteomic analysis was used to search for characteristic alterations in the sera of hepatocellular carcinoma (HCC) patients who had undergone curative radiofrequency ablation treatment. Serum samples collected from eight patients before and after treatment were subjected to 2-DE. Eighty-eight protein spots differentially expressed with the treatment were selected by clustering analysis, and the proteins were identified by MS based on MALDI-TOF/TOF analysis and public database searches. The statistical analysis suggested that four proteins decreased after treatment (pro-apolipoprotein, α2-HS glycoprotein, apolipoprotein A-IV precursor, and PRO1708/PRO2044, which is the carboxy terminal fragment of albumin) and that seven proteins were increased after treatment, including leucine-rich α2-glycoprotein and α1-antitrypsin. These data facilitate the identification of differentially expressed proteins that are involved in HCC carcinogenesis and provide candidate biomarkers for the development of diagnostic and therapeutic tools.

Original languageEnglish
Pages (from-to)4287-4295
Number of pages9
Issue number16
StatePublished - Nov 2005
Externally publishedYes


  • 2-DE
  • Hepatocellular carcinoma
  • Serum proteome


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