TY - JOUR
T1 - Proteomic analysis of Schistosoma mansoni egg secretions
AU - Cass, Cynthia L.
AU - Johnson, Jeffrey R.
AU - Califf, Lindsay L.
AU - Xu, Tao
AU - Hernandez, Hector J.
AU - Stadecker, Miguel J.
AU - Yates, John R.
AU - Williams, David L.
N1 - Funding Information:
The authors thank Dr. Tim Day for the gift of anti-PHM antiserum and Dr. Philip Lo Verde for the infected hamster liver. This work was funded by NIH/NIAID grant R03AI059037. Schistosome materials for this work were supplied in part through NIH-NIAID contract N01-AI-55270.
PY - 2007/10
Y1 - 2007/10
N2 - Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced by egg antigens. In this study, using Multidimensional Protein Identification Technology, we identified the Schistosoma mansoni egg secretome consisting of 188 proteins. Notably we identified proteins involved in redox balance, molecular chaperoning and protein folding, development and signaling, scavenging and metabolic pathways, immune response modulation, and 32 novel, previously uncharacterized schistosome proteins. We localized a subset of previously characterized schistosome proteins identified in egg secretions in this study, to the surface of live S. mansoni eggs using the circumoval precipitin reaction. The identification of proteins actively secreted by live schistosome eggs provides important new information for understanding immune modulation and the pathology of schistosomiasis.
AB - Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced by egg antigens. In this study, using Multidimensional Protein Identification Technology, we identified the Schistosoma mansoni egg secretome consisting of 188 proteins. Notably we identified proteins involved in redox balance, molecular chaperoning and protein folding, development and signaling, scavenging and metabolic pathways, immune response modulation, and 32 novel, previously uncharacterized schistosome proteins. We localized a subset of previously characterized schistosome proteins identified in egg secretions in this study, to the surface of live S. mansoni eggs using the circumoval precipitin reaction. The identification of proteins actively secreted by live schistosome eggs provides important new information for understanding immune modulation and the pathology of schistosomiasis.
KW - Egg secreted proteins
KW - Egg secretome
KW - MudPIT proteomics
KW - Schistosoma mansoni
UR - http://www.scopus.com/inward/record.url?scp=34547775859&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2007.06.002
DO - 10.1016/j.molbiopara.2007.06.002
M3 - Article
C2 - 17644200
AN - SCOPUS:34547775859
SN - 0166-6851
VL - 155
SP - 84
EP - 93
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 2
ER -