Protein biochip-based semiquantitative detection for plasma leptin

Lei Ye, Yuan Hu, Fei Hong Xu, Cheng Yun Cai, Da Wei Song, Zhen Shan Xu, Wei Dong Du

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Purpose: Plasma leptin is secreted from adipose tissues and plays pivotal roles in human physiological and pathological processes. Here, we aimed at conducting a protein biochip-based sandwich-like approach for detection of plasma leptin among healthy individuals, obesity, and diabetes patients. Experimental design: Totally, 96 plasma samples, including 45 healthy individuals with standard body mass index (BMI), 28 obesity and 23 diabetes patients, were recruited in the study. Plasma leptin was detected by a well-established protein biochip. Meanwhile an ELISA was also performed for assessment of the leptin detection by the protein biochip. Results: We found that the plasma leptin level in the obesity and diabetes patients was significantly higher than that in healthy individuals with standard body mass index (p < 0.001). The limit detection concentration of leptin was as low as 0.006 μg/mL. The plasma leptin could be semiquantitatively detected by the protein biochip. The compatibility of the biochip-based detection approach seemed acceptable in comparison with the ELISA assay (R2 = 0.948). Conclusions: We provided a protein biochip-based approach for plasma detection. This approach would be a potential substitution for the ELISA assay.

Original languageEnglish
Article number1600073
JournalProteomics - Clinical Applications
Volume11
Issue number5-6
DOIs
StatePublished - May 2017
Externally publishedYes

Keywords

  • Diabetes
  • Enzyme-linked immunosorbent assay
  • Leptin
  • Obesity
  • Protein biochip

Fingerprint

Dive into the research topics of 'Protein biochip-based semiquantitative detection for plasma leptin'. Together they form a unique fingerprint.

Cite this